Prolactin receptors in the rat hypothalamus: autoradiographic localization and characterization

Détails

ID Serval
serval:BIB_5A95AC559249
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Prolactin receptors in the rat hypothalamus: autoradiographic localization and characterization
Périodique
Neuroendocrinology
Auteur⸱e⸱s
Crumeyrolle-Arias  M., Latouche  J., Jammes  H., Djiane  J., Kelly  P. A., Reymond  M. J., Haour  F.
ISSN
0028-3835
Statut éditorial
Publié
Date de publication
03/1993
Peer-reviewed
Oui
Volume
57
Numéro
3
Pages
457-66
Notes
Comparative Study
Journal Article --- Old month value: Mar
Résumé
A precise mapping of prolactin (PRL) receptors in the rat brain has been achieved. Localization of binding sites for both 125I-human growth hormone (125I-hGH) and 125I-monoclonal anti-PRL receptor (125I-U5) was studied by in vitro autoradiography on brain sections in female rats (n = 7). The analysis of autoradiograms generated from 12 adjacent sections at 11 different brain levels (bregma 0.2 to -4.8 mm) revealed 9 distinctive localizations for 125I-hGH binding sites: preoptic suprachiasmatic nucleus, medial preoptic area, periventricular, supraoptic, paraventricular, arcuate and vetromedial nuclei and also the median eminence and the infundibulum. Specificity for PRL binding was assessed by competition experiment of 125I-hGH with unlabeled hGH and ovine PRL. Binding sites were similarly localized by 125I-U5 indicating the presence of PRL receptors moiety. The quantitative analysis with 0.6 nM 125I-hGH demonstrated maximal densities in the preoptic suprachiasmatic and arcuate nuclei and minimal densities in the median eminence and the infundibulum. Due to ample antero-posterior variations no significant changes were observed during the estrous cycle. Saturation analysis of binding in the arcuate nucleus indicated a single class of high affinity (Kd from 0.9 to 2.2 nM) receptors (Bmax from 34 to 44 fmol/mg of proteins). The present data provide the hypothalamic cartography of PRL receptors in the female rat brain and support all the physiological evidence for the existence of a direct action of PRL in the hypothalamus.
Mots-clé
Animals Arcuate Nucleus/chemistry Autoradiography Binding, Competitive Female Growth Hormone/metabolism Humans Hypothalamus/*chemistry Iodine Radioisotopes Median Eminence/chemistry Paraventricular Hypothalamic Nucleus/chemistry Preoptic Area/chemistry Prolactin/metabolism Rats Receptors, Prolactin/*analysis/metabolism Supraoptic Nucleus/chemistry Tissue Distribution Ventromedial Hypothalamic Nucleus/chemistry
Pubmed
Web of science
Création de la notice
11/02/2008 14:11
Dernière modification de la notice
20/08/2019 14:13
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