Cytometric assessment of mitochondria using fluorescent probes

Détails

ID Serval
serval:BIB_590CAE43502C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Cytometric assessment of mitochondria using fluorescent probes
Périodique
Cytometry A
Auteur⸱e⸱s
Cottet-Rousselle C., Ronot X., Leverve X., Mayol J. F.
ISSN
1552-4930 (Electronic)
ISSN-L
1552-4922
Statut éditorial
Publié
Date de publication
06/2011
Volume
79
Numéro
6
Pages
405-25
Langue
anglais
Notes
Cottet-Rousselle, Cecile
Ronot, Xavier
Leverve, Xavier
Mayol, Jean-Francois
eng
Review
2011/05/20
Cytometry A. 2011 Jun;79(6):405-25. doi: 10.1002/cyto.a.21061.
Résumé
Mitochondria are most important organelles in the survival of eukaryotic aerobic cells because they are the primary producers of ATP, regulators of ion homeostasis or redox state, and producers of free radicals. The key role of mitochondria in the generation of primordial ATP for the survival and proliferation of eukaryotic cells has been proven by extensive biochemical studies. In this context, it is crucial to understand the complexity of the mitochondrial compartment and its functionality and to develop experimental tools allowing the assessment of its nature and its function and metabolism. This review covers the role of the mitochondria in the cell, focusing on its structure, the mechanism of the mitochondrial respiratory chain, the maintenance of the transmembrane potential and the production of reactive oxygen species. The main probes used for mitochondrial compartment monitoring are described. In addition, various applications using mitochondrial-specific probes are detailed to illustrate the potential of flow and image cytometry in the study of the mitochondrial compartment. This review contains a panel of tools to explore mitochondria and to help researchers design experiments, determine the approach to be employed, and interpret their results.
Mots-clé
Adenosine Triphosphate/biosynthesis, Animals, Electron Transport, Flow Cytometry/*methods, Fluorescence, *Fluorescent Dyes/analysis/chemistry/metabolism, Free Radicals/metabolism, Humans, Mammals, Membrane Potential, Mitochondrial, Mice, Mitochondria/*metabolism, Molecular Imaging/*methods, Oxidation-Reduction, Oxidative Stress, Reactive Oxygen Species/metabolism
Pubmed
Création de la notice
02/05/2024 10:41
Dernière modification de la notice
28/05/2024 7:10
Données d'usage