Testing human hair for flunitrazepam and 7-amino-flunitrazepam by GC/MS-NCI.

Détails

ID Serval
serval:BIB_590466CE109A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Testing human hair for flunitrazepam and 7-amino-flunitrazepam by GC/MS-NCI.
Périodique
Forensic Science International
Auteur⸱e⸱s
Cirimele V., Kintz P., Staub C., Mangin P.
ISSN
0379-0738[print], 0379-0738[linking]
Statut éditorial
Publié
Date de publication
1997
Volume
84
Numéro
1-3
Pages
189-200
Langue
anglais
Résumé
To validate information on flunitrazepam use, we investigated human hair for flunitrazepam and its major metabolite 7-amino-flunitrazepam by gas chromatography coupled to mass spectrometry in negative chemical ionization mode of detection. Samples were twice decontaminated with methylene chloride, pulverized in a ball mill and 50 mg of powdered hair were incubated in Soerensen buffer (pH 7.6) in the presence of diazepam-d5 used as internal standard. After liquid-liquid extraction of the incubation medium with diethylether-chloroform (80:20, by vol.), the organic phase was evaporated and the dry extract was derivatizated with heptafluorobutyric anhydride. Benzodiazepines were separated on a 30 m capillary column and detected using single ion monitoring. Among 40 hair samples tested (obtained from drug addicts deceased by heroin overdose), 14 were positive for both flunitrazepam and 7-amino-flunitrazepam and 12 for 7-amino-flunitrazepam only. Concentrations ranged from 31 to 129 pg/mg (mean: 60 pg/mg) and from 3 to 161 pg/mg (mean: 46 pg/mg) for flunitrazepam (14 cases) and 7-amino-flunitrazepam (26 cases), respectively. This first report described the detection of flunitrazepam and 7-amino-flunitrazepam in hair of chronic abusers. Due to the low concentrations observed, negative chemical ionization appears to be the alternative to test flunitrazepam and other benzodiazepines in hair.
Mots-clé
Flunitrazepam/analogs & derivatives, Flunitrazepam/analysis, GABA Modulators/analysis, Gas Chromatography-Mass Spectrometry/methods, Hair/chemistry, Humans, Substance Abuse Detection/methods
Pubmed
Création de la notice
19/10/2010 17:09
Dernière modification de la notice
20/08/2019 15:12
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