Exosomes release by dendritic cells: new quantification method using lasser scanning microscopy and image restoration

Détails

ID Serval
serval:BIB_58BB64C1436E
Type
Actes de conférence (partie): contribution originale à la littérature scientifique, publiée à l'occasion de conférences scientifiques, dans un ouvrage de compte-rendu (proceedings), ou dans l'édition spéciale d'un journal reconnu (conference proceedings).
Sous-type
Abstract (résumé de présentation): article court qui reprend les éléments essentiels présentés à l'occasion d'une conférence scientifique dans un poster ou lors d'une intervention orale.
Collection
Publications
Titre
Exosomes release by dendritic cells: new quantification method using lasser scanning microscopy and image restoration
Titre de la conférence
15th Annual Congress of the European Respiratory Society (ERS)
Auteur⸱e⸱s
Obregon C., Nicod L.P., Gehr P., Rothen-Rutishauser L.
Adresse
Copenhagen, Denmark, September 17-21, 2005
ISBN
0903-1936
Statut éditorial
Publié
Date de publication
2005
Peer-reviewed
Oui
Volume
26
Série
European Respiratory Journal
Pages
32S
Langue
anglais
Notes
Dendritic cells (DCs) realize a surveillance network in the lung. It has been shown that they could release small vesicles, called exosomes that might spread processed antigens or provide danger signals. The membranes of DCs were stained with either fluorescent probe DiO (green) or DiI (red). LPS-stimulated and unstimulated cells were co-cultured in a collagen matrix to trap the exosomes, and their release, size and incorporation were quantified after 6 h and 24 h. Cells were investigated by laser scanning microscopy and analyzed using the surpass module of the IMARIS software where confocal data could be segmented into individual objects allowing separation of the cell body from external objects (exosomes). When unstimulated cells were mixed with stimulated cells, in an early time (6h) unstimulated DCs incorporated exosomes into their cell membranes shown by the incorporation of red material, resulting in 25000 co-localized voxels compared to 7700 co-localized voxels in control cultures. In the same co-culture condition unstimulated cells released 21(SD 18) exosomes at 6 h and 442(SD 276) at 24 h, while unstimulated co-cultured cells released only 7(SD4) and 88(SD 64) exosomes respectively. Overall, DCs were able to release numerous exosomes in response to danger signals, and in an early stage they were able to integrate neighboring DCs' exosomes providing activation signals. Using the surpass module we were able to identify and quantify exosomes.
Création de la notice
31/03/2010 12:44
Dernière modification de la notice
20/08/2019 15:12
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