Infection of CD8+ T lymphocytes with HIV. Requirement for interaction with infected CD4+ cells and induction of infectious virus from chronically infected CD8+ cells

Détails

ID Serval
serval:BIB_58AE6E1B4938
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Infection of CD8+ T lymphocytes with HIV. Requirement for interaction with infected CD4+ cells and induction of infectious virus from chronically infected CD8+ cells
Périodique
Journal of Immunology
Auteur(s)
De Maria  A., Pantaleo  G., Schnittman  S. M., Greenhouse  J. J., Baseler  M., Orenstein  J. M., Fauci  A. S.
ISSN
0022-1767 (Print)
Statut éditorial
Publié
Date de publication
04/1991
Volume
146
Numéro
7
Pages
2220-6
Notes
In Vitro
Journal Article
Research Support, U.S. Gov't, P.H.S. --- Old month value: Apr 1
Résumé
In this study, we have investigated the basic requirements for HIV-1 infection of CD8+ lymphocytes in vitro. Unfractionated PBL obtained from healthy HIV-1 seronegative donors were activated with PHA and infected in vitro with HIV-1LAV. Based on immunofluorescent labeling, the vast majority of cells (85 to 97%) surviving peak virus replication belonged to the CD8+ subset and only a small percentage (0.5 to 1.5%) were CD4+. Amplification of HIV-1 proviral sequences by polymerase chain reaction performed on the sorted surviving CD8+ cells demonstrated that CD8+ cells harbored HIV-1 proviral DNA. In addition, stimulation of these HIV-1-infected, CD8(+)-sorted cells either with PHA or anti-CD2 mAb resulted in induction of virus replication, as measured by reverse transcriptase activity. Electron microscopic analysis of CD8+ cells chronically infected with HIV-1 and stimulated with PHA showed typical virions budding from, and associated with, the surface of cells immunolabeled with gold beads directed toward the CD8 molecule. Infection of CD8+ cells with HIV-1 occurred only when CD4+ cells were present in the PHA-activated lymphocyte population exposed to HIV-1 at the beginning of the culture or when sorted CD8+CD4- lymphocytes were cocultured with autologous sorted CD8-CD4+ cells that had been previously infected with HIV-1. Coculture of these cells with PHA-blasts and incubation of their supernatants with a CD4+ cell line showed that these chronically infected CD8+ cells could spread HIV-1 infection to uninfected CD4+ cells after stimulation with PHA or anti-CD2 mAb. Therefore, these results suggest that the minimal requirement for in vitro infection of CD3+CD8+CD4- lymphocytes is the presence of infected CD4+ cells and that infected CD8+ T lymphocytes can further spread the infection to CD4+ cells.
Mots-clé
Antigens, CD/analysis Antigens, CD3 Antigens, CD8 Antigens, Differentiation, T-Lymphocyte/*analysis CD4-Positive T-Lymphocytes/*microbiology Cells, Cultured DNA, Viral/analysis Flow Cytometry HIV Infections/*microbiology HIV-1/*growth & development Humans Microscopy, Electron Polymerase Chain Reaction RNA-Directed DNA Polymerase/analysis Receptors, Antigen, T-Cell/analysis T-Lymphocyte Subsets/*microbiology Virus Replication
Pubmed
Web of science
Création de la notice
25/01/2008 15:14
Dernière modification de la notice
20/08/2019 14:12
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