Monoclonal antibody against carcinoembryonic antigen (CEA) identifies two new forms of crossreacting antigens of molecular weight 90,000 and 160,000 in normal granulocytes.

Détails

ID Serval
serval:BIB_58905FD29A86
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Monoclonal antibody against carcinoembryonic antigen (CEA) identifies two new forms of crossreacting antigens of molecular weight 90,000 and 160,000 in normal granulocytes.
Périodique
Molecular Immunology
Auteur(s)
Audette M., Buchegger F., Schreyer M., Mach J.P.
ISSN
0161-5890 (Print)
ISSN-L
0161-5890
Statut éditorial
Publié
Date de publication
1987
Peer-reviewed
Oui
Volume
24
Numéro
11
Pages
1177-1186
Langue
anglais
Résumé
Two new forms of non-specific crossreacting antigens (NCAs) were identified in the Nonidet P40 (NP-40) extracts of normal granulocytes by precipitation with the monoclonal antibody (MAb) 192 directed against carcinoembryonic antigen (CEA) and already known to crossreact with the perchloric acid soluble NCA-55. The NP-40 soluble NCAs recognized by MAb 192 have apparent mol. wts of 90,000 and 160,000 in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Both NCAs appear to consist of a single monomeric polypeptide chain, since they have the same electrophoretic mobility in SDS-PAGE under reduced and non-reduced conditions. When granulocytes were extracted with perchloric acid instead of NP-40, only the 55,000 mol. wt antigen, corresponding to the previously described NCA-55, was precipitated by MAb 192. Furthermore, it was shown that NCA-55 is not a degradation product of NCA-90 or NCA-160 due to the perchloric acid treatment because exposure to perchloric acid of NCA preparations purified from NP-40 extracts did not change their apparent mol. wts in SDS-PAGE. It was also shown that NCA-160 is not a granulocytic form of CEA because it was not precipitated by the MAb 35 reacting exclusively with CEA. Immunocytochemical studies of granulocytes and macrophages showed that MAb 192 stained both types of cells whereas MAb 47 stained only the granulocytes and MAb 35 none of these cells. In granulocytes both MAbs reacted with antigens associated with granules and also present at the periphery of the nucleus as well as in the Golgi apparatus. The NCA-90 identified by MAb 192 was found by sequential immunodepletion to be antigenically distinct from the NCA-95 precipitated by MAb 47. The epitope recognized by MAb 192 on CEA and NCA molecules appears to be on the peptidic moiety because the antigens deglycosylated by the enzyme Endo F were still precipitated by this MAb. Taken together, the results indicate that MAb 192 identifies two novel forms of NCA (NCA-90 and NCA-160) in NP-40 extracts of granulocytes, which are distinct from CEA and the previously described NCA-55 and NCA-95 identified by MAbs 192 and 47, respectively, in perchloric acid extracts of granulocytes.
Mots-clé
Antibodies, Monoclonal/diagnostic use, Antigens/analysis, Antigens/isolation & purification, Antigens, Neoplasm, Carcinoembryonic Antigen/immunology, Cell Adhesion Molecules, Cross Reactions, Electrophoresis, Polyacrylamide Gel, Glycoproteins/immunology, Glycoproteins/isolation & purification, Granulocytes/immunology, Humans, Immunoenzyme Techniques, Macrophages/immunology, Molecular Weight
Pubmed
Web of science
Création de la notice
25/01/2008 12:28
Dernière modification de la notice
20/08/2019 15:12
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