The oxidation-state-dependent ATP-binding site of cytochrome c. Implication of an essential arginine residue and the effect of occupancy on the oxidation-reduction potential

Détails

ID Serval
serval:BIB_581A83805BB0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
The oxidation-state-dependent ATP-binding site of cytochrome c. Implication of an essential arginine residue and the effect of occupancy on the oxidation-reduction potential
Périodique
Biochemical Journal
Auteur(s)
Corthesy  B. E., Wallace  C. J.
ISSN
0264-6021 (Print)
Statut éditorial
Publié
Date de publication
06/1988
Volume
252
Numéro
2
Pages
349-55
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun 1
Résumé
Arg-91 is not part of the active site of cytochrome c that mediates binding and electron transfer, yet it is absolutely conserved in eukaryotic cytochromes c, indicating a special function. The physicochemical properties of analogues are unaffected by the modification of this residue, so they can be used with confidence to study the role of Arg-91. We have established limiting conditions under which this residue alone is specifically modified by cyclohexane-1,2-dione, and have subsequently shown that ATP, and to a lesser extent ADP or Pi, protects it from the action of the reagent in an oxidation-state-dependent manner. These observations strongly support the idea that this site exerts a controlling influence on cytochrome c activity in the electron transport or other cellular redox systems, and we have commenced a study of how that influence might operate. We find that the redox potentials of both cytochrome c and analogue are little affected by changing ATP or Pi concentrations.
Mots-clé
Adenosine Triphosphate/*metabolism Amino Acids/analysis Arginine/*metabolism Binding Sites/drug effects Chromatography, Ion Exchange Cyclohexanones/pharmacology Cytochrome c Group/*metabolism Electrophoresis, Paper Oxidation-Reduction
Pubmed
Web of science
Création de la notice
25/01/2008 15:53
Dernière modification de la notice
20/08/2019 15:11
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