Quantification of endogenous Angiotensin 1-10, 1-9, 1-8, 1-7, and 1-5 in human plasma using micro-UHPLC-MS/MS: Outlining the importance of the pre-analytics for reliable results.

Détails

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Etat: Public
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_581711601DE7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Quantification of endogenous Angiotensin 1-10, 1-9, 1-8, 1-7, and 1-5 in human plasma using micro-UHPLC-MS/MS: Outlining the importance of the pre-analytics for reliable results.
Périodique
Journal of pharmaceutical and biomedical analysis
Auteur⸱e⸱s
Maurer J., de Groot A., Martin L., Grouzmann E., Wuerzner G., Eugster P.J.
ISSN
1873-264X (Electronic)
ISSN-L
0731-7085
Statut éditorial
Publié
Date de publication
15/06/2024
Peer-reviewed
Oui
Volume
243
Pages
116101
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Angiotensin peptides (ANGs) play a central role in the renin-angiotensin-aldosterone system, rendering them interesting biomarkers associated with hypertension. Precise quantification of circulating ANGs holds the potential to assess the activity of angiotensin-converting enzyme (ACE), a key protease targeted by widely prescribed drugs, namely ACE inhibitors. This ability could pave the way for personalised medicine, offering insights into the prescription of inhibitors targeting either the proteases or the receptors within the system. Despite recent developments in liquid chromatography-mass spectrometry (LC-MS) methods for measuring circulating ANG concentrations, comprehensive stability studies of ANGs in human plasma are absent in the literature, raising concerns about the reliability of measured concentrations and their link to clinical conditions. To address this critical gap, we conducted an exhaustive evaluation of the pre-analytical stability of ANG1-10, ANG1-9, ANG1-8, ANG1-7, and ANG1-5. By employing surfactants to mitigate non-specific adsorption and a dedicated mix of protease inhibitors to limit protease activity, we established an MS-based assay for these five peptides. We used this method to quantify circulating concentrations of ANGs in the plasma of 11 healthy donors and 3 patients under kidney dialysis. Our findings revealed that ANG1-10 and ANG1-8 circulate at concentrations ranging from 1 to 10 pM in healthy subjects and exhibit a high degree of correlation. Notably, ANG1-9, ANG1-7, and ANG1-5 were undetectable in any of the 14 patients, despite a sub-picomolar limit of detection. This strikingly contrasts with the reference concentrations reported in the literature, which typically fall within the picomolar range. In light of these discrepancies, we strongly advocate for rigorous pre-analytical considerations and comprehensive stability studies to ensure reliable results. We emphasise the pivotal role of heightened pre-analytical awareness within the clinical chemistry community, and we hope for continued growth in this critical area.
Mots-clé
Humans, Tandem Mass Spectrometry/methods, Chromatography, High Pressure Liquid, Reproducibility of Results, Angiotensins, Peptides, ACE, Angiotensin, Hypertension, Mass spectrometry, Preanalytical stability
Pubmed
Web of science
Open Access
Oui
Création de la notice
22/03/2024 14:37
Dernière modification de la notice
18/05/2024 6:58
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