Functional similarities between human immunodeficiency virus type 1 and simian virus 40 kappa B proto-enhancers.

Détails

ID Serval
serval:BIB_58154E6AEF62
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Functional similarities between human immunodeficiency virus type 1 and simian virus 40 kappa B proto-enhancers.
Périodique
Journal of Virology
Auteur(s)
Phares W., Herr W.
ISSN
0022-538X[print], 0022-538X[linking]
Statut éditorial
Publié
Date de publication
05/1991
Volume
65
Numéro
5
Pages
2200-2210
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
To search for broadly active enhancer elements within the human immunodeficiency virus type 1 (HIV-1) long terminal repeat, we have used a proto-enhancer amplification assay. In this assay, the enhancer region of simian virus 40 (SV40) is replaced by heterologous regulatory sequences. Upon passage in African green monkey kidney cells. SV40 growth revertants can arise by amplification (usually duplication) of active protoenhancers within the heterologous sequences. Most of the HIV-1 U3 regulatory sequences were assayed; only amplification of one or both of the HIV-1 enhancer core kappa B motifs consistently resulted in viable SV40 virus. Examination of the cell-specific enhancer activity of the individual HIV-1 kappa B proto-enhancers showed that, like the broadly active SV40 kappa B proto-enhancer (C proto-enhancer), they are all active in noninduced cell lines of either lymphoid (H9 and Jurkat) or nonlymphoid (HeLa and CV-1) origin. Unexpectedly, one of three kappa B point mutants that exhibit little or no activity in unstimulated cells is as highly induced in stimulated Jurkat cells as are the wild-type kappa B proto-enhancers. This point mutation shows that kappa B-related proto-enhancers can display markedly different activation properties in unstimulated cells yet still activate transcription to similar levels in stimulated cells.
Mots-clé
Animals, Base Sequence, Binding Sites, Cell Line, DNA, Viral, Enhancer Elements, Genetic, Gene Amplification, HIV Long Terminal Repeat/genetics, HIV-1/genetics, Humans, Immunoglobulin kappa-Chains/genetics, Immunoglobulin kappa-Chains/metabolism, Molecular Sequence Data, Mutation, NF-kappa B/metabolism, Sequence Homology, Nucleic Acid, Simian virus 40/genetics, Vero Cells
Pubmed
Web of science
Création de la notice
24/01/2008 16:36
Dernière modification de la notice
20/08/2019 15:11
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