Oviposition by pierid butterflies triggers defense responses in Arabidopsis.

Détails

ID Serval
serval:BIB_57E1F0166855
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Oviposition by pierid butterflies triggers defense responses in Arabidopsis.
Périodique
Plant Physiology
Auteur(s)
Little D., Gouhier-Darimont C., Bruessow F., Reymond P.
ISSN
0032-0889[print], 0032-0889[linking]
Statut éditorial
Publié
Date de publication
2007
Volume
143
Numéro
2
Pages
784-800
Langue
anglais
Résumé
Insect eggs represent a threat for the plant as hatching larvae rapidly start with their feeding activity. Using a whole-genome microarray, we studied the expression profile of Arabidopsis (Arabidopsis thaliana) leaves after oviposition by two pierid butterflies. For Pieris brassicae, the deposition of egg batches changed the expression of hundreds of genes over a period of 3 d after oviposition. The transcript signature was similar to that observed during a hypersensitive response or in lesion-mimic mutants, including the induction of defense and stress-related genes and the repression of genes involved in growth and photosynthesis. Deposition of single eggs by Pieris rapae caused a similar although much weaker transcriptional response. Analysis of the jasmonic acid and salicylic acid mutants coi1-1 and sid2-1 indicated that the response to egg deposition is mostly independent of these signaling pathways. Histochemical analyses showed that egg deposition is causing a localized cell death, accompanied by the accumulation of callose, and the production of reactive oxygen species. In addition, activation of the pathogenesis-related1::beta-glucuronidase reporter gene correlated precisely with the site of egg deposition and was also triggered by crude egg extract. This study provides molecular evidence for the detection of egg deposition by Arabidopsis plants and suggests that oviposition causes a localized response with strong similarity to a hypersensitive response.
Mots-clé
Animals, Apoptosis, Arabidopsis/metabolism, Arabidopsis Proteins/genetics, Arabidopsis Proteins/metabolism, Butterflies/physiology, Cyclopentanes/metabolism, Female, Gene Expression Profiling, Gene Expression Regulation, Plant, Oviposition/physiology, Ovum/physiology, Ovum/ultrastructure, Oxylipins, Plant Leaves/anatomy & histology, Plant Leaves/metabolism, Salicylic Acid/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 20:46
Dernière modification de la notice
20/08/2019 15:11
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