Helicase-defective RuvB(D113E) promotes RuvAB-mediated branch migration in vitro.

Détails

ID Serval
serval:BIB_57D378A4F7BD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Helicase-defective RuvB(D113E) promotes RuvAB-mediated branch migration in vitro.
Périodique
Journal of Molecular Biology
Auteur(s)
George H., Mézard C., Stasiak A., West S.C.
ISSN
0022-2836[print], 0022-2836[linking]
Statut éditorial
Publié
Date de publication
1999
Volume
293
Numéro
3
Pages
505-519
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
In Escherichia coli, the RuvA and RuvB proteins interact at Holliday junctions to promote branch migration leading to the formation of heteroduplex DNA. RuvA provides junction-binding specificity and RuvB drives ATP-dependent branch migration. Since RuvB contains sequence motifs characteristic of a DNA helicase and RuvAB exhibit helicase activity in vitro, we have analysed the role of DNA unwinding in relation to branch migration. A mutant RuvB protein, RuvB(D113E), mutated in helicase motif II (the DExx box), has been purified to homogeneity. The mutant protein forms hexameric rings on DNA similar to those formed by wild-type protein and promotes branch migration in the presence of RuvA. However, RuvB(D113E) exhibits reduced ATPase activity and is severely compromised in its DNA helicase activity. Models for RuvAB-mediated branch migration that invoke only limited DNA unwinding activity are proposed.
Mots-clé
Adenosine Triphosphatases/chemistry, Adenosine Triphosphatases/genetics, Adenosine Triphosphate/metabolism, Amino Acid Motifs, Amino Acid Sequence, Amino Acid Substitution, Bacterial Proteins/chemistry, Bacterial Proteins/genetics, DNA/chemistry, DNA/genetics, DNA Helicases/chemistry, DNA Helicases/genetics, DNA, Single-Stranded/chemistry, DNA, Single-Stranded/genetics, DNA, Superhelical/chemistry, DNA, Superhelical/genetics, DNA-Binding Proteins/metabolism, Escherichia coli/enzymology, Escherichia coli/genetics, Escherichia coli Proteins, Genes, Bacterial/genetics, Genes, Bacterial/physiology, Kinetics, Models, Genetic, Molecular Sequence Data, Mutation, Nucleic Acid Conformation, Nucleic Acid Heteroduplexes/chemistry, Nucleic Acid Heteroduplexes/genetics, Phenotype, Recombination, Genetic/genetics, Ultraviolet Rays
Pubmed
Web of science
Création de la notice
24/01/2008 11:36
Dernière modification de la notice
20/08/2019 15:11
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