VanT, a homologue of Vibrio harveyi LuxR, regulates serine, metalloprotease, pigment, and biofilm production in Vibrio anguillarum.

Détails

ID Serval
serval:BIB_57842DEA514E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
VanT, a homologue of Vibrio harveyi LuxR, regulates serine, metalloprotease, pigment, and biofilm production in Vibrio anguillarum.
Périodique
Journal of bacteriology
Auteur(s)
Croxatto A., Chalker V.J., Lauritz J., Jass J., Hardman A., Williams P., Cámara M., Milton D.L.
ISSN
0021-9193 (Print)
ISSN-L
0021-9193
Statut éditorial
Publié
Date de publication
03/2002
Peer-reviewed
Oui
Volume
184
Numéro
6
Pages
1617-1629
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Vibrio anguillarum possesses at least two N-acylhomoserine lactone (AHL) quorum-sensing circuits, one of which is related to the luxMN system of Vibrio harveyi. In this study, we have cloned an additional gene of this circuit, vanT, encoding a V. harveyi LuxR-like transcriptional regulator. A V. anguillarum Delta vanT null mutation resulted in a significant decrease in total protease activity due to loss of expression of the metalloprotease EmpA, but no changes in either AHL production or virulence. Additional genes positively regulated by VanT were identified from a plasmid-based gene library fused to a promoterless lacZ. Three lacZ fusions (serA::lacZ, hpdA-hgdA::lacZ, and sat-vps73::lacZ) were identified which exhibited decreased expression in the Delta vanT strain. SerA is similar to 3-phosphoglycerate dehydrogenases and catalyzes the first step in the serine-glycine biosynthesis pathway. HgdA has identity with homogentisate dioxygenases, and HpdA is homologous to 4-hydroxyphenylpyruvate dioxygenases (HPPDs) involved in pigment production. V. anguillarum strains require an active VanT to produce high levels of an L-tyrosine-induced brown color via HPPD, suggesting that VanT controls pigment production. Vps73 and Sat are related to Vibrio cholerae proteins encoded within a DNA locus required for biofilm formation. A V. anguillarum Delta vanT mutant and a mutant carrying a polar mutation in the sat-vps73 DNA locus were shown to produce defective biofilms. Hence, a new member of the V. harveyi LuxR transcriptional activator family has been characterized in V. anguillarum that positively regulates serine, metalloprotease, pigment, and biofilm production.
Mots-clé
Biofilms/growth & development, Blotting, Northern, Carbohydrate Dehydrogenases, Gene Expression Regulation, Bacterial, Genes, Bacterial, Metalloendopeptidases/biosynthesis, Metalloendopeptidases/genetics, Molecular Sequence Data, Mutagenesis, Phosphoglycerate Dehydrogenase, Pigments, Biological/biosynthesis, Pigments, Biological/genetics, Racemases and Epimerases/genetics, Serine/biosynthesis, Serine/genetics, Transcription, Genetic, Vibrio/chemistry, Vibrio/genetics
Pubmed
Web of science
Création de la notice
24/03/2019 12:51
Dernière modification de la notice
20/08/2019 14:11
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