BMP signaling components are expressed in human fracture callus

Détails

ID Serval
serval:BIB_572A95AC6E3A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
BMP signaling components are expressed in human fracture callus
Périodique
Bone
Auteur⸱e⸱s
Kloen  P., Di  P. M., Borens  O., Richmond  J., Perino  G., Helfet  D. L., Goumans  M. J.
ISSN
8756-3282 (Print)
Statut éditorial
Publié
Date de publication
2003
Volume
33
Numéro
3
Pages
362-371
Notes
DA - 20030918
LA - eng
PT - Journal Article
PT - Research Support, Non-U.S. Gov't
RN - 0 (Bone Morphogenetic Proteins)
RN - 0 (DNA-Binding Proteins)
RN - 0 (Procollagen)
RN - 0 (Receptors, Growth Factor)
RN - 0 (Smad Proteins)
RN - 0 (Trans-Activators)
RN - 0 (Transforming Growth Factor beta)
RN - 0 (bone morphogenetic protein 2)
RN - 0 (bone morphogenetic protein 3)
RN - 0 (bone morphogenetic protein 4)
RN - 0 (bone morphogenetic protein 7)
RN - EC 2.7.1.37 (BMPR1A protein, human)
RN - EC 2.7.1.37 (BMPR2 protein, human)
RN - EC 2.7.1.37 (Bone Morphogenetic Protein Receptors, Type I)
RN - EC 2.7.1.37 (Bone Morphogenetic Protein Receptors, Type II)
RN - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
SB - IM
Résumé
Of the various growth factors involved in the healing response after a fracture, bone morphogenetic proteins (BMPs) are emerging as key modulators. BMPs exert their effects by binding to a complex of type I and type II receptors leading to the phosphorylation of specific downstream effector proteins called Smads. The current study examined the presence of BMP signaling components in human callus obtained from five nascent malunions undergoing fracture fixation. These callus samples represented various stages of bone healing and a mixture of endochondral and intramembraneous bone healing. We performed immunohistochemistry on the callus, using antibodies for BMP (BMP-2,-3,-4,-7), their receptors (BMPR-IA, -IB, -II), and phosphorylated BMP receptor-regulated Smads (pBMP-R-Smads). Active osteoblasts showed fairly consistent positive staining for all BMPs that were examined, with the immunoreactivity most intense for BMP-7 and BMP-3. Immunostaining for BMPs in osteoblasts appeared to colocalize with the expression of BMPR-IA, -IB, and -II. Positive immunostaining for pBMP-R-Smads suggests that the BMP receptors expressed in these cells are activated. Staining for BMPs in cartilage cells was variable. The immunostaining appeared stronger in more mature cells, whereas staining for BMP receptors in cartilage cells was less ubiquitous. However, the expression of pBMP-R-Smads in cartilage cells suggests active signal transduction. Fibroblast-like cells also had a variable staining pattern. Overall, our findings indicate the presence of BMPs, their various receptors, and activated forms of receptor-regulated Smads in human fracture callus. To the best of our knowledge, this is the first study that documents the expression of these proteins in human fracture tissue. Complete elucidation of the roles of BMP in bone formation will hopefully lead to improved fracture healing care
Mots-clé
Adolescent/Adult/Bone Morphogenetic Protein Receptors,Type I/Bone Morphogenetic Protein Receptors,Type II/Bone Morphogenetic Proteins/Bony Callus/DNA-Binding Proteins/Fracture Fixation/Fracture Healing/Fractures,Bone/Humans/Immunohistochemistry/Male/metabolism/physiology/Procollagen/Protein-Serine-Threonine Kinases/Receptors,Growth Factor/Smad Proteins/surgery/Trans-Activators/Transforming Growth Factor beta
Pubmed
Web of science
Création de la notice
25/02/2008 12:59
Dernière modification de la notice
20/08/2019 14:11
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