REF proteins mediate the export of spliced and unspliced mRNAs from the nucleus.

Détails

ID Serval
serval:BIB_56EEDF04C6CD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
REF proteins mediate the export of spliced and unspliced mRNAs from the nucleus.
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur(s)
Rodrigues J.P., Rode M., Gatfield D., Blencowe B.J., Carmo-Fonseca M., Izaurralde E.
ISSN
0027-8424 (Print)
ISSN-L
0027-8424
Statut éditorial
Publié
Date de publication
2001
Volume
98
Numéro
3
Pages
1030-1035
Langue
anglais
Résumé
The REF family of evolutionarily conserved heterogeneous ribonucleoprotein (hnRNP)-like proteins consists of one central RNP-type RNA binding domain flanked by Arg-Gly-rich regions of variable length. Members of this protein family bind directly to RNA and the mRNA export factor TAP/Mex67p, and it has been suggested that they facilitate the recruitment of TAP/Mex67p to cellular mRNPs. We show that the variable regions are necessary for binding of REFs to RNA and to TAP. Antibodies specific to REFs prevent their interaction with RNA in vitro. After microinjection into Xenopus oocytes, these antibodies inhibit mRNA nuclear export. This inhibition of export is observed whether or not the mRNAs are generated by splicing. The antibodies do not interfere with pre-mRNA splicing or with the nuclear export of constitutive transport element (CTE)-containing RNAs (directly mediated by TAP), so REF proteins must play a critical role in mRNA nuclear export, acting downstream of splicing and upstream of TAP/Mex67p. We also show that recombinant REFs stimulate directly the export of mRNAs that are otherwise exported inefficiently. Together, our data indicate that REFs are directly implicated in the export of mRNAs from the nucleus. More generally, we show that spliced and unspliced mRNAs use common export factors to reach the cytoplasm.
Mots-clé
Amino Acid Sequence, Animals, Cell Nucleus/metabolism, Conserved Sequence, Heterogeneous-Nuclear Ribonucleoproteins, Oocytes/physiology, Protein Binding, RNA Splicing, RNA, Messenger/genetics, RNA, Messenger/metabolism, Recombinant Fusion Proteins/metabolism, Recombinant Proteins/metabolism, Ribonucleoproteins/chemistry, Ribonucleoproteins/metabolism, Xenopus laevis
Pubmed
Web of science
Open Access
Oui
Création de la notice
12/12/2012 12:21
Dernière modification de la notice
20/08/2019 15:11
Données d'usage