Optimisation of lithium-substituted bioactive glasses to tailor cell response for hard tissue repair.

Détails

ID Serval
serval:BIB_56E81895033A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Optimisation of lithium-substituted bioactive glasses to tailor cell response for hard tissue repair.
Périodique
Journal of materials science
Auteur⸱e⸱s
da Silva J.G., Babb R., Salzlechner C., Sharpe P.T., Brauer D.S., Gentleman E.
ISSN
0022-2461 (Print)
ISSN-L
0022-2461
Statut éditorial
Publié
Date de publication
2017
Peer-reviewed
Oui
Volume
52
Numéro
15
Pages
8832-8844
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Bioactive glasses (BG) are used clinically because they can both bond to hard tissue and release therapeutic ions that can stimulate nearby cells. Lithium has been shown to regulate the Wnt/β-catenin cell signalling pathway, which plays important roles in the formation and repair of bone and teeth. Lithium-releasing BG, therefore, have the potential to locally regulate hard tissue formation; however, their design must be tailored to induce an appropriate biological response. Here, we optimised the release of lithium from lithium-substituted BG by varying BG composition, particle size and concentration to minimise toxicity and maximise upregulation of the Wnt target gene Axin2 in in vitro cell cultures. Our results show that we can tailor lithium release from BG over a wide therapeutic and non-toxic range. Increasing the concentration of BG in cell culture medium can induce toxicity, likely due to modulations in pH. Nevertheless, at sub-toxic concentrations, lithium released from BG can upregulate the Wnt pathway in 17IA4 cells, similarly to treatment with LiCl. Taken together, these data demonstrate that ion release from lithium-substituted BG can be tailored to maximise biological response. These data may be important in the design of BG that can regulate the Wnt/β-catenin pathway to promote hard tissue repair or regeneration.
Pubmed
Web of science
Open Access
Oui
Création de la notice
12/01/2024 10:14
Dernière modification de la notice
13/01/2024 7:10
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