Functional analysis in vitro and in vivo of Mycobacterium bovis strain BCG-specific T cell clones

Détails

ID Serval
serval:BIB_556133773AAF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Functional analysis in vitro and in vivo of Mycobacterium bovis strain BCG-specific T cell clones
Périodique
Journal of Immunology
Auteur(s)
Pedrazzini  T., Louis  J. A.
ISSN
0022-1767 (Print)
Statut éditorial
Publié
Date de publication
03/1986
Volume
136
Numéro
5
Pages
1828-34
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar 1
Résumé
Several cloned T cell lines specific for PPD and BCG were obtained. All clones were able to secrete lymphokine, i.e., MAF/interferon, upon antigenic stimulation. The surface phenotype of all these different clones was Thy-1.2+, L3T4+, Lyt-2-, suggesting that these lines belonged to the helper/inducer T cell subset. The T cell clones displayed various degrees of helper activity as tested in a secondary antibody response in vitro. The capacity of these clones to elicit DTH reactions in the presence of antigen and their ability to inhibit mycobacterial growth in vivo were tested by transferring locally the different clones to normal mice. The clones which exhibited little or no helper activity were able to elicit DTH responses, whereas the clone with strong helper activity did not. Both types of functionally defined clones had the capacity to inhibit the growth of intracellular mycobacteria in vivo.
Mots-clé
Animals Clone Cells/immunology/metabolism/transplantation Epitopes/immunology Female Hypersensitivity, Delayed/immunology Immunization, Passive Interferons/biosynthesis Lymphocyte Activation Lymphokines/biosynthesis Macrophage-Activating Factors Mice Mice, Inbred C57BL Mycobacterium bovis/growth & development/*immunology Phenotype Species Specificity T-Lymphocytes/*immunology/metabolism/transplantation T-Lymphocytes, Helper-Inducer/immunology/metabolism Tuberculin/*immunology
Pubmed
Web of science
Création de la notice
25/01/2008 9:45
Dernière modification de la notice
20/08/2019 15:10
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