Cholesterol is a critical cellular component for T-lymphocyte cytotoxicity

Détails

ID Serval
serval:BIB_552727618C91
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Cholesterol is a critical cellular component for T-lymphocyte cytotoxicity
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur(s)
Heiniger  H. J., Brunner  K. T., Cerottini  J. C.
ISSN
0027-8424 (Print)
Statut éditorial
Publié
Date de publication
11/1978
Volume
75
Numéro
11
Pages
5683-7
Notes
Journal Article
Research Support, U.S. Gov't, P.H.S. --- Old month value: Nov
Résumé
Preincubation of cytolytic T lymphocytes (CTLs) generated in secondary C57BL/6 anti-DBA/2 mixed leukocyte cultures with an inhibitor of cellular cholesterol synthesis (25-OH-cholesterol) for 24 hr strongly depressed the cytolytic activity as determined in a 3-hr 51Cr assay. The effect of the inhibitor was reversed by the simultaneous addition of cholesterol or of mevalonic acid during the preincubation period (mevalonate is the product of the regulatory enzyme in the sterol synthesis pathway, 3-hydroxy-3-methylglutaryl-CoA reductase (NADP) [mevalonate:NADP+ oxidoreductase (CoA-acylating), EC 1.1.1.34]). Because, under the same culture conditions, inhibition of DNA synthesis had no effect on CTL activity, the experiments suggest that the effect of 25-OH-cholesterol is related to its inhibitory effect on sterol synthesis, resulting in decreased levels of membrane-bound cholesterol, rather than to inhibition of cellular proliferation.
Mots-clé
Animals Cells, Cultured Cholesterol/*physiology *Cytotoxicity, Immunologic/drug effects Hydroxycholesterols/pharmacology Mice T-Lymphocytes/drug effects/*immunology/physiology
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/01/2008 12:14
Dernière modification de la notice
20/08/2019 15:09
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