Cell-mediated immunity to antigens associated with murine sarcoma virus-induced tumors: augmentation of cytolytic T lymphocyte activity by successive specific and nonspecific stimulation in vitro

Détails

ID Serval
serval:BIB_5516DDC65BE4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Cell-mediated immunity to antigens associated with murine sarcoma virus-induced tumors: augmentation of cytolytic T lymphocyte activity by successive specific and nonspecific stimulation in vitro
Périodique
European Journal of Immunology
Auteur⸱e⸱s
Ryser  J. E., Cerottini  J. C., Brunner  K. T.
ISSN
0014-2980 (Print)
Statut éditorial
Publié
Date de publication
03/1979
Volume
9
Numéro
3
Pages
179-84
Notes
Journal Article --- Old month value: Mar
Résumé
It has been shown previously that lymphoid cells from mice which have rejected a tumor induced by the Moloney sarcoma virus-leukemia virus (MSV-MLV) complex develop high levels of specific H-2-restricted cytolytic T lymphocyte (CTL) activity after in vitro stimulation with syngeneic, irradiated MLV-induced lymphoma cells in mixed leukocyte-tumor cell cultures (MLTC). Attempts to further increase lytic activity by restimulating long-term MLTC cells with syngeneic, irradiated lymphoma cells have met but with limited success. This report shows that, in contrast to the lack of increased activity observed after specific stimulation with lymphoma cells, nonspecific stimulation of long-term MLTC cells either with supernatants from secondary mixed leukocyte cultures (2 degrees MLC SN) or with supernatants from concanavalin A-stimulated spleen cells leads, on a per cell basis, to a further 5 to 10-fold increase in CTL activity. The stimulatory activity of 2 degrees MLC SN is due to a factor(s) of apparent mol. wt. of 25,000 to 40,000. The activity of the CTL populations formed under these conditions is at least 100-fold higher against syngeneic as compared to allogeneic MLV-induced or unrelated tumor cells.
Mots-clé
Animals *Antigens, Neoplasm *Cytotoxicity, Immunologic Female *Immunity, Cellular Kinetics Leukemia Virus, Murine Lymphocyte Culture Test, Mixed Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Inbred DBA Moloney murine leukemia virus Sarcoma, Experimental/*immunology Spleen/immunology T-Lymphocytes/*immunology Time Factors
Pubmed
Web of science
Création de la notice
28/01/2008 12:13
Dernière modification de la notice
20/08/2019 15:09
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