Triplex real-time PCR method for the qualitative detection of European and American foulbrood in honeybee.

Détails

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ID Serval
serval:BIB_54A80CC0E581
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
UNIL/CHUV
Titre
Triplex real-time PCR method for the qualitative detection of European and American foulbrood in honeybee.
Périodique
Journal of microbiological methods
Auteur⸱e⸱s
Dainat B., Grossar D., Ecoffey B., Haldemann C.
ISSN
1872-8359 (Electronic)
ISSN-L
0167-7012
Statut éditorial
Publié
Date de publication
03/2018
Peer-reviewed
Oui
Volume
146
Pages
61-63
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The bacteria Melissococcus plutonius and Paenibacillus larvae, causative agents of respectively European and American foulbrood, damage honeybee health worldwide. Here, we present a specific and sensitive qualitative triplex real-time PCR method to detect simultaneously those microbial agents and a honeybee gene, validated through a study involving 7 laboratories through Europe.
Mots-clé
Americas, Animal Diseases/diagnosis, Animal Diseases/microbiology, Animals, Bees/genetics, Bees/microbiology, DNA, Bacterial/genetics, Enterococcaceae/genetics, Enterococcaceae/isolation & purification, Enterococcaceae/pathogenicity, Europe, Multiplex Polymerase Chain Reaction/methods, Multiplex Polymerase Chain Reaction/veterinary, Paenibacillus larvae/genetics, Paenibacillus larvae/isolation & purification, Paenibacillus larvae/pathogenicity, Apis mellifera, Diagnosis, Melissococcus plutonius, Multiplex real-time PCR, Paenibacillus larvae
OAI-PMH
oai:serval.unil.ch:BIB_54A80CC0E581
DOI
10.1016/j.mimet.2018.01.018
Pubmed
29391178
Web of science
000428490300013
Création de la notice
12/04/2018 11:23
Dernière modification de la notice
20/08/2019 15:09
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