Epidermal growth factor (EGF) stimulation of cultured brain cells. I. Enhancement of the developmental increase in glial enzymatic activity.

Détails

ID Serval
serval:BIB_549B8C2F16EA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Epidermal growth factor (EGF) stimulation of cultured brain cells. I. Enhancement of the developmental increase in glial enzymatic activity.
Périodique
Developmental Brain Research
Auteur(s)
Honegger P., Guentert-Lauber B.
ISSN
0165-3806
Statut éditorial
Publié
Date de publication
1983
Volume
11
Numéro
2
Pages
245-251
Langue
anglais
Résumé
Serum-free aggregating cell cultures of fetal rat telencephalon grown in the presence of 3 ng/ml (5 X 10(-10) M) epidermal growth factor (EGF) until day 12 showed 2- to 3-fold increased activities in the two glial enzymes, glutamine synthetase (GLU-S) and 2',3'-cyclic nucleotide 3'-phosphohydrolase (CNPase). This effect was concentration-dependent, with maximal stimulation in cultures treated daily with 3 ng/ml EGF. Addition of EGF during the first 10 culture days was sufficient to produce a maximal stimulation of both GLU-S and CNPase on day 19, whereas treatments starting on day 12 were ineffective. The stimulation of GLU-S preceded that of CNPase. The EGF-induced increase in GLU-S activity was not directly dependent on the presence of insulin, triiodothyronine, or hydrocortisone in the medium, whereas insulin was required for the stimulation of CNPase. A single dose of 5 ng/ml EGF on day 2 caused a slight but significant decrease in DNA synthesis after day 6. The present results indicate that in serum-free aggregating cell cultures of fetal rat telencephalon EGF partially inhibits DNA synthesis, and stimulates an early step in glial differentiation.
Mots-clé
2',3'-Cyclic Nucleotide 3'-Phosphodiesterase, 2',3'-Cyclic-Nucleotide Phosphodiesterases/metabolism, Animals, Cell Aggregation, Cell Differentiation/drug effects, Cells, Cultured, DNA/biosynthesis, Epidermal Growth Factor/pharmacology, Fetus, Glutamate-Ammonia Ligase/metabolism, Neuroglia/enzymology, Phosphoric Diester Hydrolases/metabolism, Rats, Rats, Inbred Strains, Telencephalon/drug effects
Pubmed
Web of science
Création de la notice
24/01/2008 14:12
Dernière modification de la notice
20/08/2019 15:09
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