Heterologous expression and regulation of the lysA genes of Pseudomonas aeruginosa and Escherichia coli.

Détails

ID Serval
serval:BIB_546DE5CC30B8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Heterologous expression and regulation of the lysA genes of Pseudomonas aeruginosa and Escherichia coli.
Périodique
Molecular and General Genetic
Auteur⸱e⸱s
Martin C., Cami B., Borne F., Jeenes D.J., Haas D., Patte J.C.
ISSN
0026-8925 (Print)
ISSN-L
0026-8925
Statut éditorial
Publié
Date de publication
1986
Volume
203
Numéro
3
Pages
430-434
Langue
anglais
Résumé
The Pseudomonas aeruginosa lysA gene encoding diaminopimelate decarboxylase (DAP-decarboxylase) was cloned into a broad host range vector. This gene complemented a lys mutation at the lys-12 locus of P. aeruginosa and a lysA defect in Escherichia coli. The P. aeruginosa DAP-decarboxylase was synthesized constitutively in P. aeruginosa as well as in E. coli, where the Pseudomonas lysA gene was poorly expressed. By contrast, the E. coli lysA gene was expressed well in P. aeruginosa and subject to lysine regulation when the E. coli LysR activator protein was provided. This indicates that the mechanism of transcriptional activation for the E. coli lysA gene is effective in the heterologous host.
Mots-clé
Bacterial Proteins, Carboxy-Lyases/genetics, Cloning, Molecular, Conjugation, Genetic, DNA Restriction Enzymes, Escherichia coli/genetics, Genes, Genes, Bacterial, Genetic Vectors, Genotype, Plasmids, Pseudomonas aeruginosa/enzymology, Pseudomonas aeruginosa/genetics
Pubmed
Web of science
Création de la notice
25/01/2008 17:01
Dernière modification de la notice
20/08/2019 14:09
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