Anti-myelin-associated glycoprotein antibodies in patients with a monoclonal IgM gammopathy and polyneuropathy, and a simplified method for the preparation of glycolipid antigens.

Détails

ID Serval
serval:BIB_54647E33E3DD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Anti-myelin-associated glycoprotein antibodies in patients with a monoclonal IgM gammopathy and polyneuropathy, and a simplified method for the preparation of glycolipid antigens.
Périodique
Journal of immunological methods
Auteur(s)
Burger D., Perruisseau G., Steck A.J.
ISSN
0022-1759 (Print)
ISSN-L
0022-1759
Statut éditorial
Publié
Date de publication
24/06/1991
Peer-reviewed
Oui
Volume
140
Numéro
1
Pages
31-36
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The two sulfated glucuronic acid containing glycolipids, sulfate-3-glucuronyl paragloboside (SGPG) and sulfate-3-glucuronyl lactosaminyl paragloboside (SGLPG), were prepared by ion exchange chromatography of lipid extracts from bovine cauda equina. Thin layer chromatography (TLC) immunostaining and an enzyme-linked immunosorbent assay (ELISA) were used to show that the SGPG/SGLPG fraction was free of crossreactive antigenic components such as gangliosides. The results obtained by ELISA demonstrate that sera of all patients were validated IgM monoclonal anti-myelin-associated glycoprotein (MAG) demyelinating neuropathy display high titer antibodies with high affinity type titration curves. These antibodies were absent from the sera of 16 patients with other neurological diseases and ten normal controls. ELISA with SGPG/SGLPG as an antigen appears to be a valuable and highly specific immunodiagnostic test for determining anti-MAG antibody titers in the sera of patients. The amount (approximately 0.4 mg) of SGPG/SGLPG partially purified from 10 g of fresh tissue is sufficient for the preparation of approximately 100 96-well plates for ELISA procedures.
Mots-clé
Chromatography, Ion Exchange, Chromatography, Thin Layer, Enzyme-Linked Immunosorbent Assay, Globosides/immunology, Humans, Hypergammaglobulinemia/immunology, Immunoglobulin M, Myelin Proteins/analysis, Myelin Proteins/immunology, Myelin-Associated Glycoprotein, Nervous System Diseases/immunology
Pubmed
Web of science
Création de la notice
06/08/2018 11:38
Dernière modification de la notice
20/08/2019 15:09
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