Thy-1-mediated cell-cell contact induces astrocyte migration through the engagement of αVβ3 integrin and syndecan-4.

Détails

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Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_54240D40F73D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Thy-1-mediated cell-cell contact induces astrocyte migration through the engagement of αVβ3 integrin and syndecan-4.
Périodique
Biochimica et Biophysica Acta. Molecular Cell Research
Auteur(s)
Kong M., Muñoz N., Valdivia A., Alvarez A., Herrera-Molina R., Cárdenas A., Schneider P., Burridge K., Quest A.F., Leyton L.
ISSN
0167-4889
ISSN-L
1879-2596
Statut éditorial
Publié
Date de publication
2013
Peer-reviewed
Oui
Volume
1833
Numéro
6
Pages
1409-1420
Langue
anglais
Résumé
Cell adhesion to the extracellular matrix proteins occurs through interactions with integrins that bind to Arg-Gly-Asp (RGD) tripeptides, and syndecan-4, which recognizes the heparin-binding domain of other proteins. Both receptors trigger signaling pathways, including those that activate RhoGTPases such as RhoA and Rac1. This sequence of events modulates cell adhesion to the ECM and cell migration. Using a neuron-astrocyte model, we have reported that the neuronal protein Thy-1 engages αVβ3 integrin and syndecan-4 to induce RhoA activation and strong astrocyte adhesion to their underlying substrate. Thus, because cell-cell interactions and strong cell attachment to the matrix are considered antagonistic to cell migration, we hypothesized that Thy-1 stimulation of astrocytes should preclude cell migration. Here, we studied the effect of Thy-1 expressing neurons on astrocyte polarization and migration using a wound-healing assay and immunofluorescence analysis. Signaling molecules involved were studied by affinity precipitation, western blotting and the usage of specific antibodies. Intriguingly, Thy-1 interaction with its two receptors was found to increase astrocyte polarization and migration. The latter events required interactions of these receptors with both the RGD-like sequence and the heparin-binding domain of Thy-1. Additionally, prolonged Thy-1-receptor interactions inhibited RhoA activation while activating FAK, PI3K and Rac1. Therefore, sustained engagement of integrin and syndecan-4 with the neuronal surface protein Thy-1 induces astrocyte migration. Interestingly we identify here, a cell-cell interaction that despite initially inducing strong cell attachment, favors cell migration upon persistent stimulation by engaging the same signaling receptors and molecules as those utilized by the extracellular matrix proteins to stimulate cell movement.
Mots-clé
Thy-1, Integrin, Syndecan-4, Neuron-astrocyte interaction, Cell migration
Pubmed
Web of science
Open Access
Oui
Création de la notice
06/06/2013 9:43
Dernière modification de la notice
20/08/2019 15:09
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