NrsZ: a novel, processed, nitrogen-dependent, small non-coding RNA that regulates Pseudomonas aeruginosa PAO1 virulence.

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Etat: Public
Version: Final published version
ID Serval
serval:BIB_53BD1DDECAE6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
NrsZ: a novel, processed, nitrogen-dependent, small non-coding RNA that regulates Pseudomonas aeruginosa PAO1 virulence.
Périodique
Environmental Microbiology
Auteur⸱e⸱s
Wenner N., Maes A., Cotado-Sampayo M., Lapouge K.
ISSN
1462-2920 (Electronic)
ISSN-L
1462-2912
Statut éditorial
Publié
Date de publication
2014
Volume
16
Numéro
4
Pages
1053-1068
Langue
anglais
Résumé
The opportunistic pathogen Pseudomonas aeruginosa PAO1 has a remarkable capacity to adapt to various environments and to survive with limited nutrients. Here, we report the discovery and characterization of a novel small non-coding RNA: NrsZ (nitrogen-regulated sRNA). We show that under nitrogen limitation, NrsZ is induced by the NtrB/C two component system, an important regulator of nitrogen assimilation and P. aeruginosa's swarming motility, in concert with the alternative sigma factor RpoN. Furthermore, we demonstrate that NrsZ modulates P. aeruginosa motility by controlling the production of rhamnolipid surfactants, virulence factors notably needed for swarming motility. This regulation takes place through the post-transcriptional control of rhlA, a gene essential for rhamnolipids synthesis. Interestingly, we also observed that NrsZ is processed in three similar short modules, and that the first short module encompassing the first 60 nucleotides is sufficient for NrsZ regulatory functions.
Mots-clé
Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Gene Expression Regulation, Bacterial, Glycolipids/metabolism, Nitrogen/metabolism, Pseudomonas aeruginosa/pathogenicity, Pseudomonas aeruginosa/physiology, RNA, Small Untranslated/chemistry, RNA, Small Untranslated/genetics, Virulence/genetics, Virulence Factors/genetics, beta-Galactosidase/genetics, beta-Galactosidase/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
29/01/2014 11:05
Dernière modification de la notice
20/08/2019 14:08
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