Human T cell recognition of cloned HLA class I gene products expressed on DNA transfectants of mouse mastocytoma P815

Détails

ID Serval
serval:BIB_53B93F00A945
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Human T cell recognition of cloned HLA class I gene products expressed on DNA transfectants of mouse mastocytoma P815
Périodique
European Journal of Immunology
Auteur(s)
Maryanski  J. L., Moretta  A., Jordan  B., De Plaen  E., Van Pel  A., Boon  T., Cerottini  J. C.
ISSN
0014-2980 (Print)
Statut éditorial
Publié
Date de publication
11/1985
Volume
15
Numéro
11
Pages
1111-7
Notes
Journal Article --- Old month value: Nov
Résumé
Cloned genes for human major histocompatibility complex (MHC) class I antigens were introduced by DNA-mediated gene transfer into a high-efficiency transfection recipient (HTR) cell line previously derived from mouse mastocytoma P815. Cell surface expression of HLA-A3, AW24 and CW3 gene products on P815 transfectants was demonstrated by radioimmune assay and by flow cytometry. The human MHC class I gene products were apparently expressed on P815 transfectants in a form recognized by human cytolytic T lymphocytes (CTL). Human CTL generated in unidirectional mixed lymphocyte culture against AW24+ donor lymphocytes clearly lysed P815-HLA-AW24+ transfectant target cells, but not untransfected P815(HTR) controls. Moreover, P815-HLA transfectants could stimulate in vitro a significant alloreactive human CTL response. Lysis of P815-HLA transfectant target cells by human CTL was inhibited by a monoclonal antibody directed against human MHC class I gene products. These mouse cell transfectants may be useful for the study of human T cell responses.
Mots-clé
Animals Antibodies, Monoclonal/physiology Binding, Competitive Cloning, Molecular Cytotoxicity, Immunologic DNA/*metabolism *Genes, MHC Class II HLA Antigens/*genetics/immunology Lymphocyte Activation Lymphocyte Culture Test, Mixed Mast-Cell Sarcoma/*genetics/immunology Mice T-Lymphocytes, Cytotoxic/immunology/*metabolism *Transfection
Pubmed
Web of science
Création de la notice
28/01/2008 12:14
Dernière modification de la notice
20/08/2019 15:08
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