Development of a small-lumen vascular prosthesis coated with autologous endothelial cells

Détails

ID Serval
serval:BIB_538BA1BFC311
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Development of a small-lumen vascular prosthesis coated with autologous endothelial cells
Périodique
Helvetica Chirurgica Acta
Auteur(s)
Lehmann  K., Muller-Glauser  W., Bittmann  P., Bay  U., Dittes  P., von Segesser  L., Turina  M.
ISSN
0018-0181
Statut éditorial
Publié
Date de publication
06/1989
Volume
56
Numéro
1-2
Pages
39-43
Notes
Comparative Study
English Abstract
Journal Article --- Old month value: Jun
Résumé
A new compliant prosthesis with a monolayer of autologous endothelial cells (ENC) has been developed. It consists of a porous polyurethane-siloxane-copolymer reinforced by a polyester network to prevent excessive dilatation. On the inner surface an ENC monolayer is established before implantation by a cell culture procedure. The prosthesis displays compliance (13.2 +/- 3.0 x 10(-4) mmHg-1) comparable to native arteries. It is non-kinkable (minimal radius of curvature less than 5 mm). Burst resistance, remaining deformation, cut out force and tensile strength are superior to standard values. ENC-coverage in excess of 95% of the inner surface was produced in vitro using a lining procedure. The monolayer of confluent cells was demonstrated to consist of endothelial cells by their characteristic cobblestone morphology, the expression of factor VIII related antigen and the specific uptake of Dil-Ac-LDL. The unstimulated prostacyclin production was similar both in native veins as well as in lined prostheses. Antithrombogenicity of the endothelial cell lining was demonstrated in 24 h animal implants.
Mots-clé
Animals *Biocompatible Materials *Blood Vessel Prosthesis Dogs Endothelium, Vascular/*transplantation Graft Occlusion, Vascular/*prevention & control Humans Models, Cardiovascular Prosthesis Design
Pubmed
Création de la notice
14/02/2008 15:16
Dernière modification de la notice
20/08/2019 15:08
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