Chief cells possess a receptor with high affinity for PACAP and VIP that stimulates pepsinogen release
Détails
ID Serval
serval:BIB_53812AE2EA75
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Chief cells possess a receptor with high affinity for PACAP and VIP that stimulates pepsinogen release
Périodique
American Journal of Physiology
ISSN
0002-9513 (Print)
Statut éditorial
Publié
Date de publication
12/1992
Volume
263
Numéro
6 Pt 1
Pages
G901-7
Notes
Journal Article --- Old month value: Dec
Résumé
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a 38-amino acid peptide of the secretin-vasoactive intestinal peptide (VIP) family. To investigate whether PACAP alters chief cell function, we prepared isolated chief cells (> 90% pure) from guinea pig stomach. PACAP-38, PACAP-27, VIP, and secretin all caused a threefold increase in pepsinogen release. The dose-response curves of PACAP-38, PACAP-27, and VIP were biphasic, whereas with secretin it was not. The first phase comprised 40% of maximal release, and each of the three peptides (PACAP-38, PACAP-27, and VIP) were equipotent (EC50 0.1-0.3 nM). For the second phase, comprising 60% of maximal release, the relative potencies were PACAP-38 > PACAP-27 = VIP. 125I-labeled secretin, 125I-VIP, and 125I-PACAP-27 all demonstrated saturable binding to chief cells. Binding of both 125I-PACAP-27 and 125I-VIP was inhibited completely and with similar potencies by PACAP-38, PACAP-27, and VIP. Secretin had a > 500-fold lower affinity than PACAP-38 for displacing both 125I-PACAP-27 and 125I-VIP. With 125I-secretin, secretin was the most potent, and was 197 times more potent than PACAP-38, which was 6-8 times more potent than both PACAP-27 and VIP. We conclude that both PACAP-38 and PACAP-27 stimulate pepsinogen secretion from dispersed chief cells. In contrast to a number of other tissues, no evidence for a high-affinity receptor that interacted only with PACAP was found. PACAP and VIP interact with equal high affinity with a common receptor and with low affinity with the secretin receptor.
Mots-clé
Animals
Binding, Competitive
Dose-Response Relationship, Drug
Guinea Pigs
Male
Neuropeptides/*metabolism/pharmacology
Pepsinogens/*metabolism
Pituitary Adenylate Cyclase-Activating Polypeptide
Receptors, Cell Surface/metabolism/*physiology
Secretin/metabolism/pharmacology
Stomach/cytology/*metabolism
Vasoactive Intestinal Peptide/*metabolism/pharmacology
Pubmed
Web of science
Création de la notice
25/01/2008 15:58
Dernière modification de la notice
20/08/2019 14:08