Suppression of short interfering RNA-mediated gene silencing by the structural proteins of hepatitis C virus.

Détails

ID Serval
serval:BIB_53483B727194
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Suppression of short interfering RNA-mediated gene silencing by the structural proteins of hepatitis C virus.
Périodique
Journal of General Virology
Auteur(s)
Ji J., Glaser A., Wernli M., Berke J.M., Moradpour D., Erb P.
ISSN
0022-1317
Statut éditorial
Publié
Date de publication
2008
Peer-reviewed
Oui
Volume
89
Numéro
Pt 11
Pages
2761-2766
Langue
anglais
Résumé
Viruses have evolved strategies to overcome the antiviral effects of the host at different levels. Besides specific defence mechanisms, the host responds to viral infection via the interferon pathway and also by RNA interference (RNAi). However, several viruses have been identified that suppress RNAi. We addressed the question of whether hepatitis C virus (HCV) suppresses RNAi, using cell lines constitutively expressing green fluorescent protein (GFP) and inducibly expressing HCV proteins. It was found that short interfering RNA-mediated GFP gene silencing was inhibited when the entire HCV polyprotein was expressed. Further studies showed that HCV structural proteins, and in particular envelope protein 2 (E2), were responsible for this inhibition. Co-precipitation assays demonstrated that E2 bound to Argonaute-2 (Ago-2), a member of the RNA-induced silencing complex, RISC. Thus, HCV E2 that interacts with Ago-2 is able to suppress RNAi.
Mots-clé
Cell Line, Tumor, Gene Expression Regulation, Viral, Gene Silencing, Genetic Markers, Green Fluorescent Proteins, Hepacivirus, Humans, Osteosarcoma, RNA, Small Interfering, Viral Proteins, Viral Structural Proteins
Pubmed
Web of science
Open Access
Oui
Création de la notice
02/03/2009 14:50
Dernière modification de la notice
20/08/2019 15:08
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