Pancreas preservation fluid microbial contamination is associated with poor islet isolation outcomes - a multi-centre cohort study

Détails

ID Serval
serval:BIB_53416377EB0B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Pancreas preservation fluid microbial contamination is associated with poor islet isolation outcomes - a multi-centre cohort study
Périodique
Transplant International
Auteur⸱e⸱s
Meier R. P. H., Andrey D. O., Sun P., Niclauss N., Bedat B., Demuylder-Mischler S., Borot S., Benhamou P. Y., Wojtusciszyn A., Buron F., Pernin N., Muller Y. D., Bosco D., van Delden C., Berney T.
ISSN
0934-0874
Statut éditorial
Publié
Date de publication
08/2018
Volume
31
Numéro
8
Pages
917-929
Langue
anglais
Notes
Gm5yd
Times Cited:9
Cited References Count:34
Résumé
The microbiological safety of islet preparations is paramount. Preservation medium contamination is frequent, and its impact on islet yield and function remains unclear. Microbiological samples collected during islet isolations from 2006 to 2016 were analyzed and correlated to isolation and allo- and autotransplantation outcomes. Microbial contamination of preservation medium was found in 64.4% of processed donor pancreases (291/452). We identified 464 microorganisms including Staphylococcus (253/464, 54.5%), Streptococcus (31/464, 6.7%), and Candida species (25/464, 5.4%). Microbial contamination was associated with longer warm and cold ischemia times and lower numbers of postpurification islet equivalents, purity, transplant rate, and stimulation index (all P<0.05). Six percent of the preparations accepted for transplantation showed microbial contamination after isolation (12/200); 9 of 12 were Candida species. Six patients were transplanted with a sample with late microbial growth discovered after the infusion. Insulin independence rate was not affected. This risk of transplanting a contaminated islets preparation was reduced by half following the implementation of an additional sampling after 24h of islet culture. Pancreas preservation fluid microbial contamination is associated with lower transplant rate and poorer invitro function, but not with changes in graft survival. Culture medium testing 1day after isolation reduces the risk of incidental transplantation with contaminated islets.
Mots-clé
islet isolation, islet transplantation, microbial contamination, type 1 diabetes mellitus, langerhans isolation, transplantation, bacterial, surveillance, endophthalmitis, keratoplasty, infections, protocol, network, impact
Web of science
Création de la notice
14/06/2021 9:59
Dernière modification de la notice
12/01/2022 11:50
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