Determination of nizatidine and two of its main metabolites in human serum using high-performance liquid chromatography.

Détails

ID Serval
serval:BIB_5201FEBCCE5D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Determination of nizatidine and two of its main metabolites in human serum using high-performance liquid chromatography.
Périodique
Journal of Chromatography
Auteur(s)
Tracqui A., Kintz P., Mangin P.
ISSN
0021-9673[print], 0021-9673[linking]
Statut éditorial
Publié
Date de publication
1990
Volume
529
Numéro
2
Pages
369-376
Langue
anglais
Résumé
A high-performance liquid chromatographic assay has been developed for the determination of nizatidine, a new histamine H2-receptor antagonist, and two of its main metabolites, N-desmethylnizatidine and nizatidine sulphoxide. Drugs were extracted with chloroform-2-propanol (90:10, v/v) from alkalinized samples of serum, using ranitidine as an internal standard. After evaporation of the extraction solvent, the residue was removed and analysed on a LiChrosorb Si60 5-microns column with a mobile phase of acetonitrile-methanol-water-ammonia solution (1000:200:20:5, v/v). The compounds were detected at 320 nm. The lower detection limits were 6-18 ng/ml at a signal-to-noise ratio of 3. This method is simple and specific, and the single-step extraction makes it rapid. It is the first high-performance liquid chromatographic assay to be described for the determination of nizatidine metabolites.
Mots-clé
Chromatography, High Pressure Liquid, Histamine H2 Antagonists/blood, Histamine H2 Antagonists/pharmacokinetics, Humans, Hydrogen-Ion Concentration, Nizatidine, Ranitidine/blood, Solvents, Spectrophotometry, Ultraviolet, Thiazoles/blood, Thiazoles/pharmacokinetics
Pubmed
Création de la notice
15/10/2010 16:10
Dernière modification de la notice
20/08/2019 15:07
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