Modulation of glial activation by astrocyte-derived protein S100B: differential responses of astrocyte and microglial cultures.

Détails

ID Serval
serval:BIB_4FCBADBC9385
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Modulation of glial activation by astrocyte-derived protein S100B: differential responses of astrocyte and microglial cultures.
Périodique
Brain Research
Auteur⸱e⸱s
Petrova T.V., Hu J., Van Eldik L.J.
ISSN
0006-8993 (Print)
ISSN-L
0006-8993
Statut éditorial
Publié
Date de publication
2000
Volume
853
Numéro
1
Pages
74-80
Langue
anglais
Résumé
The astrocyte-derived protein S100B stimulates production of inducible nitric oxide synthase and nitric oxide (NO) in astrocytes [Hu et al., 1996, J. Biol. Chem. 271:2543], but its effect on microglia is not known. In addition, S100B's ability to modulate the activity of other glial activating agents has not been defined. In this study, we compared the ability of S100B to stimulate NO in cultures of rat primary astrocytes and the BV-2 murine microglial cell line, and investigated the effect of the combined action of S100B and other stimuli known to activate glial cells. S100B itself stimulated the production of NO in astrocytes, and did not modify or potentiated only weakly the NO production induced by interleukin-1 beta, tumor necrosis factor alpha, dibutyryl cyclic AMP, zymosan A or lipid A. In contrast, S100B alone did not induce NO in BV-2 cells but strongly potentiated NO production in the presence of lipid A but not zymosan A. The deletion of eight C-terminal amino acid residues in S100B leads to a loss of the effect of S100B on microglia but not on astrocytes. These results demonstrate that responses of glial cells to extracellular S100B can vary depending on the cell type, and suggest that different structural features of S100B are important for the protein's effects on microglia and astrocytes.
Mots-clé
Amino Acid Sequence, Animals, Astrocytes/cytology, Astrocytes/drug effects, Calcium-Binding Proteins/genetics, Calcium-Binding Proteins/metabolism, Cattle, Cells, Cultured, Drug Synergism, Enzyme Induction/drug effects, Lipid A/metabolism, Lipid A/pharmacology, Lipopolysaccharides/metabolism, Lipopolysaccharides/pharmacology, Mice, Microglia/cytology, Microglia/drug effects, Molecular Sequence Data, Mutagenesis, Site-Directed, Nerve Growth Factors/genetics, Nerve Growth Factors/metabolism, Nitric Oxide/biosynthesis, Nitric Oxide Synthase/metabolism, Nitric Oxide Synthase Type II, Rats, Recombinant Proteins/pharmacology, S100 Proteins, Sensitivity and Specificity, Sequence Deletion, Zymosan/pharmacology
Pubmed
Web of science
Création de la notice
20/12/2012 15:52
Dernière modification de la notice
20/08/2019 14:05
Données d'usage