Serum albumin promotes ATP-binding cassette transporter-dependent sterol uptake in yeast.

Détails

ID Serval
serval:BIB_4F17C79A67B7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Serum albumin promotes ATP-binding cassette transporter-dependent sterol uptake in yeast.
Périodique
FEMS Yeast Research
Auteur(s)
Marek M., Silvestro D., Fredslund M.D., Andersen T.G., Pomorski T.G.
ISSN
1567-1364 (Electronic)
ISSN-L
1567-1356
Statut éditorial
Publié
Date de publication
2014
Volume
14
Numéro
8
Pages
1223-1233
Langue
anglais
Résumé
Sterol uptake in fungi is a multistep process that involves interaction between external sterols and the cell wall, incorporation of sterol molecules into the plasma membrane, and subsequent integration into intracellular membranes for turnover. ATP-binding cassette (ABC) transporters have been implicated in sterol uptake, but key features of their activity remain to be elucidated. Here, we apply fluorescent cholesterol (NBD-cholesterol) to monitor sterol uptake under anaerobic and aerobic conditions in two fungal species, Candida glabrata (Cg) and Saccharomyces cerevisiae (Sc). We found that in both fungal species, ABC transporter-dependent uptake of cholesterol under anaerobic conditions and in mutants lacking HEM1 gene is promoted in the presence of the serum protein albumin that is able to bind the sterol molecule. Furthermore, the C. glabrata ABC transporter CgAus1p expressed in S. cerevisiae requires the presence of serum or albumin for efficient cholesterol uptake. These results suggest that albumin can serve as sterol donor in ABC transporter-dependent sterol uptake, a process potentially important for growth of C. glabrata inside infected humans.
Mots-clé
aerobic exclusion, cholesterol, functional complementation, heterologous expression, pathogenic yeast, sterol scavenging
Pubmed
Web of science
Open Access
Oui
Création de la notice
08/01/2015 10:45
Dernière modification de la notice
20/08/2019 15:04
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