Malignant transformation of mouse primary keratinocytes by Harvey sarcoma virus and its modulation by surrounding normal cells.

Détails

ID Serval
serval:BIB_4ECA4836996B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Malignant transformation of mouse primary keratinocytes by Harvey sarcoma virus and its modulation by surrounding normal cells.
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur⸱e⸱s
Dotto G.P., Weinberg R.A., Ariza A.
ISSN
0027-8424 (Print)
ISSN-L
0027-8424
Statut éditorial
Publié
Date de publication
1988
Volume
85
Numéro
17
Pages
6389-6393
Langue
anglais
Résumé
The activated ras oncogene that is present in Harvey sarcoma virus is able to induce malignant transformation of pure cultures of mouse primary keratinocytes. Malignant transformation of these cells is demonstrated by their ability to form carcinomas when grafted back onto syngeneic animals. However, expression of the malignant phenotype by the ras-transformed keratinocytes is drastically inhibited by the presence of normal dermal fibroblasts. This inhibitory effect depends on the ratio of fibroblasts to keratinocytes. It can be observed with mitomycin C-treated growth-arrested dermal fibroblasts and not with other cells, such as normal keratinocytes or established fibroblasts. Thus, a cellular environment approximating normal tissue can suppress tumor formation triggered by a single oncogene.
Mots-clé
Animals, Animals, Newborn, Cell Division, Cell Transformation, Neoplastic, Cells, Cultured, Epidermis/cytology, Epidermis/transplantation, Fibroblasts/cytology, Genes, ras, Harvey murine sarcoma virus/genetics, Keratins/biosynthesis, Mice, Mice, Inbred BALB C, Sarcoma Viruses, Murine/genetics, Skin/cytology
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 15:58
Dernière modification de la notice
20/08/2019 15:04
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