Promoter-selective activation domains in Oct-1 and Oct-2 direct differential activation of an snRNA and mRNA promoter.

Détails

ID Serval
serval:BIB_4E5049B73F58
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Promoter-selective activation domains in Oct-1 and Oct-2 direct differential activation of an snRNA and mRNA promoter.
Périodique
Cell
Auteur(s)
Tanaka M., Lai J.S., Herr W.
ISSN
0092-8674[print], 0092-8674[linking]
Statut éditorial
Publié
Date de publication
02/1992
Volume
68
Numéro
4
Pages
755-767
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
The promoter specificity of transcriptional activators is generally thought to be conferred by the specificity of the DNA-binding domain, which brings the activation domain to the appropriate promoter sequence. We show here, however, that Oct-1 and Oct-2 can differentially activate transcription not through DNA binding specificity but instead through the use of promoter-selective activation domains. These distinct activation domains lead to stimulation of the U2 small nuclear RNA promoter by Oct-1 and an mRNA promoter by Oct-2. An Oct-2 variant, called Oct-2B, differs from Oct-2 by an Oct-1-related C-terminal extension that results from alternative splicing. This variant gains the ability to activate the U2 small nuclear RNA promoter. Thus, the promoter selectivity of a transcriptional activator can be changed, in this case by alternative splicing, without affecting its DNA binding specificity.
Mots-clé
DNA-Binding Proteins/genetics, Gene Expression Regulation, Hela Cells, Humans, Models, Genetic, Promoter Regions, Genetic, RNA Splicing, RNA, Messenger/genetics, RNA, Small Nuclear/genetics, Trans-Activators/genetics, Transfection
Pubmed
Web of science
Création de la notice
24/01/2008 16:36
Dernière modification de la notice
20/08/2019 15:03
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