Evolution of a SHOOTMERISTEMLESS transcription factor binding site promotes fruit shape determination.

Détails

ID Serval
serval:BIB_4E212ACDF404
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Evolution of a SHOOTMERISTEMLESS transcription factor binding site promotes fruit shape determination.
Périodique
Nature plants
Auteur⸱e⸱s
Hu Z.C., Majda M., Sun H.R., Zhang Y., Ding Y.N., Yuan Q., Su T.B.,  T.F., Gao F., Xu G.X., Smith R.S., Østergaard L., Dong Y.
ISSN
2055-0278 (Electronic)
ISSN-L
2055-0278
Statut éditorial
Publié
Date de publication
01/2025
Peer-reviewed
Oui
Volume
11
Numéro
1
Pages
23-35
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
In animals and plants, organ shape is primarily determined during primordium development by carefully coordinated growth and cell division <sup>1-3</sup> . Rare examples of post-primordial change in morphology (reshaping) exist that offer tractable systems for the study of mechanisms required for organ shape determination and diversification. One such example is morphogenesis in Capsella fruits whose heart-shaped appearance emerges by reshaping of the ovate spheroid gynoecium upon fertilization <sup>4</sup> . Here we use whole-organ live-cell imaging and single-cell RNA sequencing (scRNA-seq) analysis to show that Capsella fruit shape determination is based on dynamic changes in cell growth and cell division coupled with local maintenance of meristematic identity. At the molecular level, we reveal an auxin-induced mechanism that is required for morphological alteration and ultimately determined by a single cis-regulatory element. This element resides in the promoter of the Capsella rubella SHOOTMERISTEMLESS <sup>5</sup> (CrSTM) gene. The CrSTM meristem identity factor positively regulates its own expression through binding to this element, thereby providing a feed-forward loop at the position and time of protrusion emergence to form the heart. Independent evolution of the STM-binding element in STM promoters across Brassicaceae species correlates with those undergoing a gynoecium-to-fruit shape change. Accordingly, genetic and phenotypic studies show that the STM-binding element is required to facilitate the shape transition and suggest a conserved molecular mechanism for organ morphogenesis.
Mots-clé
Fruit/genetics, Fruit/growth & development, Transcription Factors/metabolism, Transcription Factors/genetics, Plant Proteins/genetics, Plant Proteins/metabolism, Gene Expression Regulation, Plant, Binding Sites, Meristem/genetics, Meristem/growth & development, Meristem/metabolism, Indoleacetic Acids/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
16/12/2024 16:38
Dernière modification de la notice
28/01/2025 7:06
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