CC chemokine receptor-2 is not essential for the development of antigen-induced pulmonary eosinophilia and airway hyperresponsiveness

Détails

ID Serval
serval:BIB_4D9A4644529E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
CC chemokine receptor-2 is not essential for the development of antigen-induced pulmonary eosinophilia and airway hyperresponsiveness
Périodique
Journal of Immunology
Auteur(s)
MacLean  J. A., De Sanctis  G. T., Ackerman  K. G., Drazen  J. M., Sauty  A., DeHaan  E., Green  F. H., Charo  I. F., Luster  A. D.
ISSN
0022-1767 (Print)
Statut éditorial
Publié
Date de publication
12/2000
Volume
165
Numéro
11
Pages
6568-75
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Dec 1
Résumé
Monocyte chemoattractant proteins-1 and -5 have been implicated as important mediators of allergic pulmonary inflammation in murine models of asthma. The only identified receptor for these two chemokines to date is the CCR2. To study the role of CCR2 in a murine model of Ag-induced asthma, we compared the pathologic and physiological responses of CCR2(-/-) mice with those of wild-type (WT) littermates following immunization and challenge with OVA. OVA-immunized/OVA-challenged (OVA/OVA) WT and CCR2(-/-) mice developed significant increases in total cells recovered by bronchoalveolar lavage (BAL) compared with their respective OVA-immunized/PBS-challenged (OVA/PBS) control groups. There were no significant differences in BAL cell counts and differentials (i.e., macrophages, PMNs, lymphocytes, and eosinophils) between OVA/OVA WT and CCR2(-/-) mice. Serologic evaluation revealed no significant difference in total IgE and OVA-specific IgE between OVA/OVA WT mice and CCR2(-/-) mice. Lung mRNA expression and BAL cytokine protein levels of IL-4, IL-5, and IFN-gamma were also similar in WT and CCR2(-/-) mice. Finally, OVA/OVA CCR2(-/-) mice developed increased airway hyper-responsiveness to a degree similar to that in WT mice. We conclude that following repeated airway challenges with Ag in sensitized mice, the development of Th2 responses (elevated IgE, pulmonary eosinophilia, and lung cytokine levels of IL-4 and IL5) and the development of airway hyper-responsiveness are not diminished by a deficiency in CCR2.
Mots-clé
Animals Antibody Specificity Antigens/administration & dosage/*immunology Bronchial Hyperreactivity/enzymology/genetics/*immunology Bronchoalveolar Lavage Fluid/cytology/immunology Chemotaxis, Leukocyte/genetics/immunology Cytokines/metabolism Eosinophil Peroxidase Eosinophils/enzymology Immunoglobulin E/blood Injections, Intraperitoneal Lung/metabolism Mice Mice, Inbred C57BL Mice, Knockout Ovalbumin/immunology Peroxidases/metabolism Pulmonary Eosinophilia/enzymology/genetics/*immunology RNA, Messenger/metabolism Receptors, Chemokine/deficiency/genetics/*physiology Ribonucleases
Pubmed
Web of science
Création de la notice
25/01/2008 9:52
Dernière modification de la notice
20/08/2019 14:02
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