Fine processes of Nestin-GFP-positive radial glia-like stem cells in the adult dentate gyrus ensheathe local synapses and vasculature.

Détails

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Etat: Public
Version: Author's accepted manuscript
ID Serval
serval:BIB_4D51115E83F0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Fine processes of Nestin-GFP-positive radial glia-like stem cells in the adult dentate gyrus ensheathe local synapses and vasculature.
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur(s)
Moss J., Gebara E., Bushong E.A., Sánchez-Pascual I., O'Laoi R., El M'Ghari I., Kocher-Braissant J., Ellisman M.H., Toni N.
ISSN
1091-6490 (Electronic)
ISSN-L
0027-8424
Statut éditorial
Publié
Date de publication
03/05/2016
Peer-reviewed
Oui
Volume
113
Numéro
18
Pages
E2536-45
Langue
anglais
Notes
Publication types: ARTICLE
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Adult hippocampal neurogenesis relies on the activation of neural stem cells in the dentate gyrus, their division, and differentiation of their progeny into mature granule neurons. The complex morphology of radial glia-like (RGL) stem cells suggests that these cells establish numerous contacts with the cellular components of the neurogenic niche that may play a crucial role in the regulation of RGL stem cell activity. However, the morphology of RGL stem cells remains poorly described. Here, we used light microscopy and electron microscopy to examine Nestin-GFP transgenic mice and provide a detailed ultrastructural reconstruction analysis of Nestin-GFP-positive RGL cells of the dentate gyrus. We show that their primary processes follow a tortuous path from the subgranular zone through the granule cell layer and ensheathe local synapses and vasculature in the inner molecular layer. They share the ensheathing of synapses and vasculature with astrocytic processes and adhere to the adjacent processes of astrocytes. This extensive interaction of processes with their local environment could allow them to be uniquely receptive to signals from local neurons, glia, and vasculature, which may regulate their fate.

Mots-clé
Animals, Astrocytes/cytology, Cells, Cultured, Cerebral Arteries/cytology, Cerebral Arteries/metabolism, Dentate Gyrus/cytology, Dentate Gyrus/metabolism, Green Fluorescent Proteins, Male, Mice, Mice, Transgenic, Nestin/metabolism, Neural Stem Cells/cytology, Neural Stem Cells/metabolism, Neurogenesis/physiology, Neuroglia/cytology, Neuroglia/metabolism, Neurovascular Coupling/physiology, Stem Cell Niche/physiology, Synapses/metabolism, Synapses/ultrastructure, Tissue Distribution
Pubmed
Open Access
Oui
Création de la notice
03/05/2016 16:38
Dernière modification de la notice
20/08/2019 14:02
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