Cell-specific expression of epithelial sodium channel alpha, beta, and gamma subunits in aldosterone-responsive epithelia from the rat: localization by in situ hybridization and immunocytochemistry

Détails

ID Serval
serval:BIB_4D2B901A1C9E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Cell-specific expression of epithelial sodium channel alpha, beta, and gamma subunits in aldosterone-responsive epithelia from the rat: localization by in situ hybridization and immunocytochemistry
Périodique
Journal of Cell Biology
Auteur(s)
Duc  C., Farman  N., Canessa  C. M., Bonvalet  J. P., Rossier  B. C.
ISSN
0021-9525
Statut éditorial
Publié
Date de publication
12/1994
Peer-reviewed
Oui
Volume
127
Numéro
6 Pt 2
Pages
1907-21
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec
Résumé
A highly selective, amiloride-sensitive, epithelial sodium channel from rat colon (rENaC), composed of three homologous subunits termed alpha, beta, and gamma rENaC, has been cloned by functional expression and was proposed to mediate electrogenic sodium reabsorption in aldosterone-responsive epithelia. To determine whether rENaC could account for sodium absorption in vivo, we studied the cellular localization of the sodium channel messenger RNA subunits by in situ hybridization and their cellular and subcellular distribution by immunocytochemistry in the kidney, colon, salivary, and sweat glands of the rat. In the kidney, we show that the three subunit mRNAs are specifically co-expressed in the renal distal convoluted tubules (DCT), connecting tubules (CNT), cortical collecting ducts (CCD), and outer medullary collecting ducts (OMCD), but not in the inner medullary collecting ducts (IMCD). We demonstrate co-localization of alpha, beta, and gamma subunit proteins in the apical membrane of a majority of cells of CCD and OMCD. Our data indicate that alpha, beta, and gamma subunit mRNAs and proteins are co-expressed in the distal nephron (excepting IMCD), a localization that correlates with the previously described physiological expression of amiloride-sensitive electrogenic sodium transport. Our data, however, suggest that another sodium transport protein mediates electrogenic amiloride-sensitive sodium reabsorption in IMCD. We also localized rENaC to the surface epithelial cells of the distal colon and to the secretory ducts of the salivary gland and sweat gland, providing further evidence consistent with the hypothesis that the highly selective, amiloride-sensitive sodium channel is physiologically expressed in aldosterone-responsive cells.
Mots-clé
Aldosterone/*pharmacology Amiloride/pharmacology Animals Antibody Specificity Biological Markers Biological Transport Colon/chemistry Epithelium/drug effects/metabolism Immunohistochemistry In Situ Hybridization Kidney/chemistry Protein Conformation RNA, Messenger/*isolation & purification Rats Rats, Sprague-Dawley Sodium/*metabolism Sodium Channels/drug effects/genetics/immunology/*isolation & purification Sublingual Gland/chemistry Sweat Glands/chemistry Tissue Distribution
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 13:00
Dernière modification de la notice
20/08/2019 14:01
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