Glucocorticoids regulate the expression of the stressprotein alpha B-crystallin

Détails

ID Serval
serval:BIB_4D11144CDC32
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Glucocorticoids regulate the expression of the stressprotein alpha B-crystallin
Périodique
Molecular and Cellular Endocrinology
Auteur(s)
Scheier  B., Foletti  A., Stark  G., Aoyama  A., Dobbeling  U., Rusconi  S., Klemenz  R.
ISSN
0303-7207
Statut éditorial
Publié
Date de publication
10/1996
Peer-reviewed
Oui
Volume
123
Numéro
2
Pages
187-98
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct 30
Résumé
alpha B-crystallin is a major component of the eye lens but is also found in many extralenticular tissues. In established fibroblasts it is synthesized in response to stress such as hyperthermia. Here we report that the treatment of NIH3T3 fibroblasts with the synthetic glucocorticoid hormone dexamethasone resulted in the accumulation of substantial amounts of alpha B-crystallin, alpha B-crystallin mRNA accumulated slowly and over a period of many days in response to prolonged hormone treatment. alpha B-crystallin promoter-reporter constructs were hormone responsive. A putative glucocorticoid response element (GRE) within the analysed promoter region could bind the glucocorticoid receptor as revealed from in vitro footprint analysis but is not involved in the hormone-mediated gene activation. Deletions of 5' flanking regions to position -465 relative to the transcription start allowed for full hormone responsiveness. A deletion from -465 to -389 abolish hormone-mediated gene induction. No sequence element closely resembling a classical GRE is present within that hormone-responsive region.
Mots-clé
3T3 Cells Animals Base Sequence Crystallins/genetics/*metabolism Dexamethasone/*pharmacology Gene Deletion Gene Expression Regulation/*drug effects Humans Mice Molecular Sequence Data Promoter Regions (Genetics)/genetics Sequence Alignment Transcription, Genetic/*drug effects
Pubmed
Web of science
Création de la notice
17/01/2008 16:06
Dernière modification de la notice
20/08/2019 14:01
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