Mitofusins 1/2 and ERRalpha expression are increased in human skeletal muscle after physical exercise

Détails

ID Serval
serval:BIB_4CBE8FC07F23
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Mitofusins 1/2 and ERRalpha expression are increased in human skeletal muscle after physical exercise
Périodique
Journal of Physiology
Auteur(s)
Cartoni R., Leger B., Hock M. B., Praz M., Crettenand A., Pich S., Ziltener J. L., Luthi F., Deriaz O., Zorzano A., Gobelet C., Kralli A., Russell A. P.
ISSN
0022-3751
Statut éditorial
Publié
Date de publication
2005
Peer-reviewed
Oui
Volume
567
Numéro
Pt 1
Pages
349-358
Langue
anglais
Résumé
Mitochondrial impairment is hypothesized to contribute to the pathogenesis of insulin resistance. Mitofusin (Mfn) proteins regulate the biogenesis and maintenance of the mitochondrial network, and when inactivated, cause a failure in the mitochondrial architecture and decreases in oxidative capacity and glucose oxidation. Exercise increases muscle mitochondrial content, size, oxidative capacity and aerobic glucose oxidation. To address if Mfn proteins are implicated in these exercise-induced responses, we measured Mfn1 and Mfn2 mRNA levels, pre-, post-, 2 and 24 h post-exercise. Additionally, we measured the expression levels of transcriptional regulators that control mitochondrial biogenesis and functions, including PGC-1alpha, NRF-1, NRF-2 and the recently implicated ERRalpha. We show that Mfn1, Mfn2, NRF-2 and COX IV mRNA were increased 24 h post-exercise, while PGC-1alpha and ERRalpha mRNA increased 2 h post-exercise. Finally, using in vitro cellular assays, we demonstrate that Mfn2 gene expression is driven by a PGC-1alpha programme dependent on ERRalpha. The PGC-1alpha/ERRalpha-mediated induction of Mfn2 suggests a role of these two factors in mitochondrial fusion. Our results provide evidence that PGC-1alpha not only mediates the increased expression of oxidative phosphorylation genes but also mediates alterations in mitochondrial architecture in response to aerobic exercise in humans
Mots-clé
Adult/Energy Metabolism/Estrogen Receptor alpha/Exercise/Gene Expression/genetics/GTP Phosphohydrolases/Humans/Male/Membrane Proteins/Membrane Transport Proteins/Mitochondria/Mitochondrial Proteins/Muscle,Skeletal/physiology/Promoter Regions (Genetics)/Signal Transduction
Pubmed
Web of science
Open Access
Oui
Création de la notice
05/03/2008 16:23
Dernière modification de la notice
20/08/2019 14:01
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