Characterization of monoclonal antibodies to a rat liver vasopressin receptor.

Détails

ID Serval
serval:BIB_4C985D15E6D1
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Characterization of monoclonal antibodies to a rat liver vasopressin receptor.
Périodique
Clinical and Experimental Pharmacology and Physiology
Auteur⸱e⸱s
Trinder D., Mooser V., Kelly J.M., Phillips P.A., Casley D., Johnston C.I.
ISSN
0305-1870 (Print)
ISSN-L
0305-1870
Statut éditorial
Publié
Date de publication
1991
Volume
18
Numéro
5
Pages
345-348
Langue
anglais
Notes
Publication types: Journal Article ; Research Support
Résumé
1. Balb/c mice were immunized against a vasopressin binding protein purified from rat liver. The hybrids produced from two cell fusions were screened against this receptor. Three hybrids were selected, cloned and expanded in serum-free media. The monoclonal antibodies (MoAb) secreted by these three hybrids were of the subclass IgM and were able to immunoprecipitate [125I]-labelled purified receptor. 2. All three MoAb bound to the purified solubilized receptor, crude liver and kidney membranes in a concentration-dependent manner. However, the binding of MoAb to the membranes did not inhibit the binding of [125I]-[d(CH2)5,Sar7]AVP, a selective V1 receptor radioligand, to the liver membrane-bound receptor. 3. These results suggest that the three MoAb recognize epitopes on the V1 receptor which are not denatured by solubilization, but are common to both rat liver and kidney membranes.
Mots-clé
Animals, Antibodies, Monoclonal/isolation & purification, Enzyme-Linked Immunosorbent Assay, Iodine Radioisotopes/diagnostic use, Kidney Medulla/metabolism, Liver/ultrastructure, Membranes/metabolism, Mice, Mice, Inbred BALB C, Precipitin Tests, Receptors, Angiotensin/immunology, Receptors, Vasopressin, Vasopressins/metabolism
Pubmed
Web of science
Création de la notice
13/10/2013 20:35
Dernière modification de la notice
20/08/2019 14:01
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