Identification of the hepatitis C virus RNA replication complex in Huh-7 cells harboring subgenomic replicons

Détails

ID Serval
serval:BIB_4BC05C3AB205
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Identification of the hepatitis C virus RNA replication complex in Huh-7 cells harboring subgenomic replicons
Périodique
Journal of Virology
Auteur(s)
Gosert  R., Egger  D., Lohmann  V., Bartenschlager  R., Blum  H. E., Bienz  K., Moradpour  D.
ISSN
0022-538X (Print)
Statut éditorial
Publié
Date de publication
05/2003
Volume
77
Numéro
9
Pages
5487-92
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May
Résumé
Formation of a membrane-associated replication complex, composed of viral proteins, replicating RNA, and altered cellular membranes, is a characteristic feature of plus-strand RNA viruses. Here, we demonstrate the presence of a specific membrane alteration, designated the membranous web, that contains hepatitis C virus (HCV) nonstructural proteins, as well as viral plus-strand RNA, in Huh-7 cells harboring autonomously replicating subgenomic HCV RNAs. Metabolic labeling with 5-bromouridine 5'-triphosphate in the presence of actinomycin D revealed that the membranous web is the site of viral RNA synthesis and therefore represents the replication complex of HCV.
Mots-clé
Cell Membrane/metabolism/ultrastructure/virology Hepacivirus/*genetics/metabolism/ultrastructure Humans RNA, Viral/*biosynthesis/genetics/*ultrastructure *Replicon Tumor Cells, Cultured Viral Nonstructural Proteins/metabolism *Virus Replication
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 16:05
Dernière modification de la notice
20/08/2019 13:59
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