Tape-Stripping Electrochemical Detection of Melanoma.

Détails

ID Serval
serval:BIB_4B5AE89097F4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Tape-Stripping Electrochemical Detection of Melanoma.
Périodique
Analytical chemistry
Auteur(s)
Darvishi S., Pick H., Lin T.E., Zhu Y., Li X., Ho P.C., Girault H.H., Lesch A.
ISSN
1520-6882 (Electronic)
ISSN-L
0003-2700
Statut éditorial
Publié
Date de publication
15/10/2019
Peer-reviewed
Oui
Volume
91
Numéro
20
Pages
12900-12908
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
A noninvasive electrochemical melanoma detection approach based on using adhesive tapes for collecting and fixing cells from a suspicious skin area and transferring the cells into a scanning electrochemical microscope (SECM) is presented. The adhesive layer collects the cells reproducibly and keeps them well adhered on the tape during experiments in an electrolyte solution. A melanoma biomarker, here the intracellular enzyme tyrosinase (TYR), was imaged on the tape-collected cells without further cell lysing using antibodies that were labeled with horseradish peroxidase (HRP). The HRP labels catalyzed the oxidation of a dissolved redox-active species, which was detected at a soft microelectrode, gently brushed in contact mode over the tape. The melanoma biomarker was first detected on tape-stripped samples with murine melanoma cells of different concentrations. Thereafter, increasing levels of TYR were recorded in cells that were collected from the skin of melanoma mouse models representing three different stages of tumor growth. Additionally, SECM results of tape-stripped different human melanoma cell lines were confirmed by previous studies based on traditionally fixed and permeabilized cells.
Pubmed
Web of science
Création de la notice
13/10/2019 18:31
Dernière modification de la notice
05/09/2020 6:26
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