Stimulation of hematopoiesis in vivo by recombinant bacterial murine interleukin 3.

Détails

ID Serval
serval:BIB_4B48D097842E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Stimulation of hematopoiesis in vivo by recombinant bacterial murine interleukin 3.
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur(s)
Kindler V., Thorens B., de Kossodo S., Allet B., Eliason J.F., Thatcher D., Farber N., Vassalli P.
ISSN
0027-8424 (Print)
ISSN-L
0027-8424
Statut éditorial
Publié
Date de publication
02/1986
Volume
83
Numéro
4
Pages
1001-1005
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Mouse interleukin 3 (IL-3) cDNA was cloned into a plasmid construction, allowing the synthesis of very high quantities of IL-3 in Escherichia coli. The recombinant (r) IL-3, purified to homogeneity, was active in vitro on the proliferation and differentiation of various hematopoietic progenitor cells at 1 pM. To maintain detectable blood levels of IL-3, osmotic pumps containing rIL-3 or control solutions were placed under the skin of normal and irradiated C3H/HeJ and (BALB X B10) F1 mice. The effect of IL-3 on hematopoietic progenitor cell numbers in spleen and bone marrow was evaluated 3 and 7 days later by using an in vitro clonal assay. The results demonstrated the following: (i) Doses of IL-3 infused at the rate of 2.5-5 ng per g of body weight per hr were sufficient to increase the numbers of hematopoietic progenitors in normal mice by at least 2-fold within 3 days. (ii) In mice with progenitor cell levels depressed by sublethal irradiation, 7-day treatment with IL-3 resulted in a 10-fold increase to near normal levels. (iii) The erythroid and myeloid lineages appeared to be enhanced to the same extent. (iv) Enhancement of hematopoiesis occurred primarily in spleen, but hematopoietic foci were also evident in the liver; in contrast, total cell and progenitor cell numbers were decreased in the bone marrow.
Mots-clé
Animals, Bone Marrow/drug effects, Bone Marrow/radiation effects, Bone Marrow Cells, Cloning, Molecular, DNA/genetics, DNA, Recombinant, Escherichia coli/metabolism, Hematopoiesis/drug effects, Hematopoiesis/radiation effects, Hematopoietic Stem Cells/drug effects, Hematopoietic Stem Cells/radiation effects, Infusions, Parenteral/instrumentation, Interleukin-3, Lymphokines/administration & dosage, Lymphokines/pharmacology, Mice, Mice, Inbred BALB C, Mice, Inbred C3H, Prostheses and Implants, Recombinant Proteins/administration & dosage, Recombinant Proteins/pharmacology, Spleen/cytology, Spleen/drug effects
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 14:41
Dernière modification de la notice
20/08/2019 14:59
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