Dynamic binding of Ku80, Ku70 and NF90 to the IL-2 promoter in vivo in activated T-cells
Détails
Télécharger: BIB_4B3FD321B301.P001.pdf (4043.95 [Ko])
Etat: Public
Version: de l'auteur⸱e
Etat: Public
Version: de l'auteur⸱e
ID Serval
serval:BIB_4B3FD321B301
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Dynamic binding of Ku80, Ku70 and NF90 to the IL-2 promoter in vivo in activated T-cells
Périodique
Nucleic Acids Research
ISSN
1362-4962 (Electronic)
Statut éditorial
Publié
Date de publication
2007
Volume
35
Numéro
7
Pages
2302-10
Notes
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Résumé
IL-2 gene expression in activated T-cells is initiated by chromatin remodeling at the IL-2 proximal promoter and conversion of a transcriptional repressor into a potent transcriptional activator. A purine-box regulator complex was purified from activated Jurkat T-cell nuclei based on sequence-specific DNA binding to the antigen receptor response element (ARRE)/nuclear factor of activated T-cells (NF-AT) target DNA sequence in the proximal IL-2 promoter. ARRE DNA-binding subunits were identified as NF90, NF45 and systemic lupus erythematosis autoantigens, Ku80 and Ku70. Monoclonal antibodies to Ku80, Ku70 and NF90 specifically inhibit constitutive and inducible ARRE DNA-binding activity in Jurkat T-cells. Ku80, Ku70 and NF90 bind specifically to the IL-2 gene promoter in vivo, as demonstrated by chromatin immunoprecipitation. Activation of Jurkat T-cells and mouse primary spleen cells induces binding of Ku80 and NF90 to the IL-2 promoter in vivo, and decreases binding of Ku70 to the IL-2 promoter in vivo, and these dynamic changes are inhibited by immunosuppressants cyclosporin A and triptolide. Dynamic changes in binding of Ku80, Ku70 and NF90 to the IL-2 proximal promoter in vivo correlate with chromatin remodeling and transcriptional initiation in activated T-cells.
Mots-clé
Animals
Antigens, Nuclear/*metabolism
Binding Sites
Cells, Cultured
Chromatin Immunoprecipitation
Cyclosporine/pharmacology
DNA-Activated Protein Kinase/metabolism
DNA-Binding Proteins/*metabolism
Diterpenes/pharmacology
Epoxy Compounds/pharmacology
Humans
Immunosuppressive Agents/pharmacology
Interleukin-2/*genetics
Jurkat Cells
Lymphocyte Activation
Mice
Mice, Inbred C57BL
Nuclear Factor 45 Protein/metabolism
Nuclear Factor 90 Proteins/*metabolism
Phenanthrenes/pharmacology
*Promoter Regions (Genetics)
Protein Subunits/metabolism
Response Elements
T-Lymphocytes/*immunology
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 15:53
Dernière modification de la notice
20/08/2019 14:59