Determination of the absolute handedness of knots and catenanes of DNA.

Détails

ID Serval
serval:BIB_4AAAB1FB8705
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Determination of the absolute handedness of knots and catenanes of DNA.
Périodique
Nature
Auteur⸱e⸱s
Krasnow M.A., Stasiak A., Spengler S.J., Dean F., Koller T., Cozzarelli N.R.
ISSN
0028-0836[print], 0028-0836[linking]
Statut éditorial
Publié
Date de publication
08/1983
Volume
304
Numéro
5926
Pages
559-560
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
DNA winds about itself in a right-handed or left-handed fashion at several structural levels. The double helix is generally right-handed and is given a (+) sign by convention, whereas supercoiling of the helix axis is always (-) in the cell. The winding in higher -order forms such as knots and catenanes is unknown, and this has impeded elucidation of the mechanisms of their formation and resolution by replication, recombination and topoisomerase action. We introduce here a procedure for determining the handedness of DNA winding by inspection of electron micrographs of DNA molecules coated with Escherichia coli RecA protein. We demonstrate the validity of the method and show that DNA topoisomerase I of E. coli generates an equal mixture of (+) and (-) duplex DNA knots, and that one product of recombination by resolvase of transposon Tn3 (refs 8, 9) is a catenane of uniquely (+) sign.
Mots-clé
Bacterial Proteins/diagnostic use, DNA, DNA Topoisomerases, Type I/metabolism, DNA, Superhelical, Nucleic Acid Conformation, Nucleotidyltransferases/metabolism, Rec A Recombinases, Recombination, Genetic, Transposases
Pubmed
Web of science
Création de la notice
24/01/2008 11:36
Dernière modification de la notice
20/08/2019 14:58
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