Assessment of airborne microorganisms by real-time PCR: optimistic findings and research challenges
Détails
ID Serval
serval:BIB_4A92849F1290
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Institution
Titre
Assessment of airborne microorganisms by real-time PCR: optimistic findings and research challenges
Périodique
Frontiers in Bioscience (Scholar Edition)
ISSN
1945-0524 (Electronic)
ISSN-L
1945-0516
Statut éditorial
Publié
Date de publication
2011
Peer-reviewed
Oui
Volume
3
Numéro
163
Pages
445-453
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Review Publication Status: epublish
Résumé
Most airborne microorganisms are natural components of our ecosystem. Soil, vegetation and animals, including humans, are sources for aerial release of these living or dead cells. In the past, assessment of airborne microorganisms was mainly restricted to occupational health concerns. Indeed, in several occupations, exposure to very high concentrations of non-infectious airborne bacteria and fungi, result in allergenic, toxic or irritant reactions. Recently, the threat of bioterrorism and pandemics have highlighted the urgent need to increase knowledge of bioaerosol ecology. More fundamentally, airborne bacterial and fungal communities begin to draw much more consideration from environmental microbiologists, who have neglected this area for a long time. This increased interest of scientists is to a great part due to the development and use of real-time PCR techniques to identify and quantify airborne microorganisms. Even if the advantages of the PCR technology are obvious, researchers are confronted with new problems. This review describes the methodological state of the art in bioaerosols field and emphasizes the future challenges and perspectives of the real-time PCR-based methods for airborne microorganism studies.
Mots-clé
Air Microbiology, Animals, Bacteria/genetics, Bacteria/isolation & purification, Fungi/genetics, Fungi/isolation & purification, Humans, Polymerase Chain Reaction/methods, Specimen Handling/methods, Viruses/genetics, Viruses/isolation & purification
DOI
Pubmed
Création de la notice
23/03/2011 12:25
Dernière modification de la notice
20/08/2019 14:58