The ATPase activity of RecA is needed to push the DNA strand exchange through heterologous regions.

Détails

ID Serval
serval:BIB_4961B11B01FE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
The ATPase activity of RecA is needed to push the DNA strand exchange through heterologous regions.
Périodique
EMBO Journal
Auteur⸱e⸱s
Rosselli W., Stasiak A.
ISSN
0261-4189[print], 0261-4189[linking]
Statut éditorial
Publié
Date de publication
12/1991
Volume
10
Numéro
13
Pages
4391-4396
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The role of ATP hydrolysis during the RecA-mediated recombination reaction is addressed in this paper. Recent studies indicated that the RecA-promoted DNA strand exchange between completely homologous double- and single-stranded DNA can be very efficient in the absence of ATP hydrolysis. In this work we demonstrate that the energy derived from the ATP hydrolysis is strictly needed to drive the DNA strand exchange through the regions where the interacting DNA molecules are not in a homologous register. Therefore, in addition to the role of the ATP hydrolysis in promoting the dissociation of RecA from the products of the recombination reaction, as described earlier, ATP hydrolysis also plays a crucial role in the actual process of strand exchange, provided that the lack of homologous register obstructs the process of branch migration.
Mots-clé
Adenosine Triphosphatases/metabolism, Adenosine Triphosphate/metabolism, Autoradiography, Base Sequence, DNA, Bacterial/genetics, DNA, Bacterial/metabolism, Electrophoresis, Polyacrylamide Gel, Hydrolysis, Molecular Sequence Data, Rec A Recombinases/metabolism, Recombination, Genetic, Substrate Specificity
Pubmed
Web of science
Création de la notice
24/01/2008 10:36
Dernière modification de la notice
20/08/2019 13:56
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