Optimisation of the formation and distribution of protoporphyrin IX in the urothelium: an in vitro approach.

Détails

ID Serval
serval:BIB_48FB6A6F77B4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Optimisation of the formation and distribution of protoporphyrin IX in the urothelium: an in vitro approach.
Périodique
The Journal of urology
Auteur⸱e⸱s
Marti A., Lange N., van den Bergh H., Sedmera D., Jichlinski P., Kucera P.
ISSN
0022-5347
Statut éditorial
Publié
Date de publication
1999
Peer-reviewed
Oui
Volume
162
Numéro
2
Pages
546-52
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Résumé
PURPOSE: To optimize conditions for photodynamic detection (PDD) and photodynamic therapy (PDT) of bladder carcinoma, urothelial accumulation of protoporphyrin IX (PpIX) and conditions leading to cell photodestruction were studied. MATERIALS AND METHODS: Porcine and human bladder mucosae were superfused with derivatives of 5-aminolevulinic acid (ALA). PpIX accumulation and distribution across the mucosa was studied by microspectrofluorometry. Cell viability and structural integrity were assessed by using vital dyes and microscopy. RESULTS: ALA esters, especially hexyl-ALA, accelerated and regularized urothelial PpIX accumulation and allowed for necrosis upon illumination. CONCLUSIONS: hexyl-ALA used at micromolar concentrations is the most efficient PpIX precursor for PDD and PDT.
Mots-clé
Aminolevulinic Acid, Animals, Humans, Photosensitizing Agents, Protoporphyrins, Swine, Tissue Distribution, Urothelium
Pubmed
Web of science
Création de la notice
24/01/2008 16:08
Dernière modification de la notice
20/08/2019 13:56
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