Lack of association of secretory component with IgA in J chain-deficient mice
Détails
ID Serval
serval:BIB_48A215B66110
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Lack of association of secretory component with IgA in J chain-deficient mice
Périodique
Journal of Immunology
ISSN
0022-1767 (Print)
Statut éditorial
Publié
Date de publication
07/1996
Volume
157
Numéro
2
Pages
750-4
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Jul 15
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Jul 15
Résumé
J chain has been proposed to play a role in the mucosal transport of polymeric Igs (pIg) by the polymeric Ig receptor (pIgR). We have previously reported the generation of J chain-deficient mice. These mice exhibited elevated serum IgA and depleted biliary and fecal IgA levels compared with wild-type mice. We report here that, unlike the IgA levels in bile and feces, IgA levels in local mucosal and glandular secretions were not depressed in J chain-deficient mice. Breast milk, intestinal mucosal surface, and nasal wash IgA levels in the mutant mice were similar to wild-type mice while bronchoalveolar lavage IgA levels were higher in the J chain-deficient animals. Western blot analysis with an Ab to secretory component (SC), the portion of the pIgR that remains bound to pig in secretions, and immunoprecipitation with Abs directed against IgA showed that secreted IgA was associated with SC in wild-type but not J chain-deficient mice. The IgA in wild-type secretions was polymeric while the secretions of J chain-deficient mice contained IgA monomers and other nonpolymeric IgA forms. Thus, J chain is not essential for IgA transport by intestinal, mammary, or respiratory epithelia but is necessary for the stable association of pIgA with SC. Further, we suggest that J chain-deficient IgA is transported into secretions by a different mechanism than wild-type IgA.
Mots-clé
Animals
Base Sequence
Bronchoalveolar Lavage Fluid/chemistry
Female
Immunoglobulin A/biosynthesis/*genetics
Immunoglobulin G/biosynthesis
Immunoglobulin J-Chains/chemistry/*genetics
Intestinal Mucosa/immunology
Male
Mammary Glands, Animal/secretion
Mice
Mice, Mutant Strains
Milk/immunology/secretion
Molecular Sequence Data
Nasal Mucosa/immunology/secretion
Secretory Component/*genetics
Pubmed
Web of science
Création de la notice
25/01/2008 14:53
Dernière modification de la notice
20/08/2019 13:55